Résumé
Objective: To establish a method for the determination of gastrodine in Tianma Formula Granule. Methods: HPLC was used with LiChrospherR 100 column, MeOH-phosphate solution(contain KH2PO4 and Na2HPO4 each 0.1mol/L)-water(1.5:3:95.5) as mobile phase and detection wavelength at 270nm. Results: Gastrodine showed a good linearity in the range of 1.638～14.742?g. The average recovery was 98.17 %and RSD was 1.39 %(n=5). Conclusion: This method is simple, accurate and with good reproducibility, and can be used for the quality control of Tianma Formula Granule.
Résumé
Objective: To study the quality standard of Niaoduqing Tablet, a preparation of Chinese herbal medicine. Methods: Thin layer chromatography (TLC) was used for the identification of Radix et Rhizoma Rhei, Radix Polygoni Multiflori, Radix Sophorae Flavescentis, Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong in Niaoduqing Tablet. TLC scanning was used for the determination of astragaloside IV and HPLC for determination of paeoniflorin in Niaoduqing Tablet. Results: TLC identification were highly specific. A good linearity for astragaloside IV was ranged 1.006～9.064?g and that for paeoniflorin was ranged 0.176～0.880?g. The average recovery of astragaloside IV was 97.69 %( RSD= 2.13 %) and that of paeoniflorin was 97.47 %( RSD=0.87 %). Conclusion: This method can be used for the standard of quality control of Niaoduqing Tablet.
Résumé
Objective To compare the effects of two crushing methods on dissolution rate and decocting rate of water-soluble protein in Plastrum Testudinis.Methods The dissolution rate of water-soluble protein was compared in Plastrum Testudinis processed by the traditional crushing method(passing 100-eyes screen,fine powder)and ultra-fine crushing method(passing 300-eye screen,ultra-fine powder);besides,orthogonal design was applied to study the process technical condition of decocting rate in Plastrum Testudinis.Results Water-soluble protein in fine powder of Plastrum Testudinis was hardly detected while the dissolution rate of water-soluble protein in ultra-fine powder was 51.2 %in 20 minutes and up to 70.5 %at 2 hours.Three influencing factors of fineness,decocting frequencies and decocting time were measured with orthogonal test.The results of variance analysis showed that fineness significantly influenced the decocting rate(P .05).Conclusion The ultra-fine powder technique is propitious to the increase of dissolution rate and decocting rate of water-soluble protein in Plastrum Testudinis.
Résumé
Objective: To establish the quality standard for Guangxiao Liniao Capsules (Herba Verbenae, Herba Leonuri, Rhizoma Alismatis, Radix Linderae, etc.). Methods: Herba Verbenae, Herba Leonuri, Rhizoma Alismatis, Radix Linderae were identified by TLC, and the content of stachydrine hydrochloride was determined by TLC scanning. Results: Herba Verbenae, Herba Leonuri, Rhizoma Alismatis, Radix Linderae could be identified by TLC. Stachydrine hydrochloride showed a good linear relationship at a range of 4?g～20?g, r = 0.9975. The average recovery was 97.9%, and RSD was 1.20%. Conclusion: The methods are accurate and can be used for the quality control of Guangxiao Liniao Capsules