Sociodemographic profile and evaluation of associated factors in Chronic suppurative otitis media patients reporting to tertiary care Hospital of Punjab

Introduction: Incidence of chronic suppurative otitis media has been reported varying from 2.55% to 9.25%. CSOM without prompt, proper treatment can progress to a variety of mild to life-threatening complications that can be intra temporal and intracranial. Thus, the present study was conducted to identify sociodemographic factors associated with Chronic suppurative otitis media. Materials and methods: The present study comprised of 100 patients with history of discharge from ear along with perforation. Detailed clinical examination along with history was taken as per proforma. Specific emphasis was given on the sociodemographic factors associated with Chronic suppurative otitis media. Results: The present study found that the majority of cases belonged to lower (poor) socioeconomic status comprising 59% of cases, 74% in study group belonged to rural areas and factors associated Dhingra R, Dhillon V, Monga S, Mehta AS, Kaur G, Kaur M. Sociodemographic profile and evaluation of associated factors in Chronic suppurative otitis media patients reporting to tertiary care Hospital of Punjab. IAIM, 2016; 3(6): 6-10. Page 7 with this infection were living in crowded conditions and in large family, unhygienic practices, such as bathing in contaminated ponds and rivers, unsterile ear piercing, leaning ears with aseptic things such as matchsticks, hairpins, pen-refills, etc. Conclusion: Sociodemographic profile and associated risk factors play a significant role in etiopathogenesis of CSOM. Thus, possible preventive strategies and programs to educate patients regarding knowledge of this burden of illness should be planned in developing countries like India.

An experimental design for induction of non-specific aortoarteritis.

BACKGROUND: An attempt was made to induce aortoarteritis in mice by using various antigens. METHODS AND RESULTS: The Swiss mice were immunized with eight different antigens and were grouped A to G. Group H served as control. The mice were then bled at 1st, 2nd, 4th, 6th and 8th month interval post-immunization for estimating antibody titer. Then the mice were sacrificed and the heart, aorta and kidney were taken out and processed for hematoxylin-eosin staining. There was gradual increase in the antibody titer from 1st month till 4th month within all the experimental groups (A-G), when compared with control group H. The titer started falling sharply from 6th month post-immunization. However, the control group H did not show much variation. When each individual group was compared separately with control group H, the significant statistical value was obtained. Histopathological examination revealed mild inflammation (+) in kidney by 2nd month, moderate inflammation (++) by 6th month, extensive inflammation (+++) by 8th month and alteration in the normal parenchyma of kidney by 8th month. CONCLUSIONS: The histopathological changes brought out through antigens were more pronounced by 8th month following injection of tunica media, tunica adventitia, tunica intima and aorta collagen as compared to that of standard collagen and mouse aorta injections.

Enzyme-linked immunosorbent assay and immunoblot study in Takayasu's arteritis patients.

Takayasu's arteritis or non-specific aortoarteritis is an inflammatory and stenotic disease of the aorta of questionable aetiology. Immunopathogenic mechanism, the precise nature of which is uncertain, is often suspected to be one of the basic causes of this disease. The present study was designed to estimate the antiaorta antibody titre in Takayasu's arteritis patients and to further locate the antigen in the vessel wall. Thirty clinically and angiographically proven cases of Takayasu's arteritis patients with appropriate controls were studied. Antiaorta antibody titres were estimated using Enzyme-Linked Immunosorbent Assay method. The controls included patients of vascular diseases other than Takayasu's arteritis, autoimmune diseases like rheumatoid arthritis and systemic lupus erythematosis and normal healthy individuals. Absorbance value at 492 nm at a dilution of 1:500 of the patients' sera was expressed as the antiaorta, antibody titre. There was significant difference (p < 0.005) between the mean value of the antibody titre in patients (0.471 +/- 0.073) and patients of other vascular diseases (0.209 +/- .056); autoimmune diseases (0.143 +/- .024); and, controls (0.108 +/- 0.012). Collagenase treatment of the aorta resulted in the fall of the antibody titre of aortitis patients (0.162 +/- 0.036) suggesting that the collagen might be one of the components responsible for autoantigenecity of aorta resulting in aortitis. The aortic extract was further subjected to 10 percent sodium dodecyle sulphate-polyacrylamide gel electrophoresis and immunoblot was done with Takayasu's arteritis patients' sera as well as controls' sera. The sera in 80 percent of Takayasu's arteritis patients immunoprecipitated a protein of molecular weight 45,000 (45 kilodalton) whereas only 15 percent patients of autoimmune disease group showed precipitation band though of lower molecular weight. Normal human sera gave no immunoprecipitation band. The precise nature of the antigen still needs to be identified.