Résumé
Un objetivo de esta presentación es el de analizar las peculiaridades distintivas de la enzima lipasa proveniente de diferentes fuentes: gástrica (LG), intestinal (LI)hepática (LH), lipoproteica (LLP), pero, en especial, aquella de la pancreática (LP), sobre todo en lo relativo a sus interacciones neuro-hormonales.
Sujets)
Humains , Cellules G , Oestradiol , Laparotomie , Triacylglycerol lipase , Micelles , Pancréas , Sécrétine , Somatostatine , TétragastrineRésumé
En este estudio se presenta el caso de un paciente pediátrico que luego de ser sometido a un transplante hepático con donante vivo relacionado, presentó un cuadro de hiperfosfatasemia alcalina sérica. Se pone énfasis en la importancia de la utilización de la técnica de isoelectroenfoque para determinar el origen tisular del hallazgo mencionado
Sujets)
Humains , Femelle , Nourrisson , Phosphatase alcaline/sang , Immunosuppresseurs/effets indésirables , Transplantation hépatique/effets indésirables , Canalicules biliaires , Canalicules biliaires/enzymologie , Rejet du greffon/diagnostic , Focalisation isoélectrique/tendances , Isoenzymes/sang , Tests de la fonction hépatique/méthodesRésumé
Myeloperoxidase (MPO), a specific polymorphonuclear leukocyte enzyme, has been used previously to quantify the number of neutrophils in tissue. MPO activity was found to be linearly related to the number of neutrophil cells. In an attempt to use this method in leukocytes measuring in stool, fecal MPO was solubilized with hexadecyltrimethylammonium bromide and the MPO activity was measured by a dianisidine-H2O2 assay. Stools from 10 normal subjects and 39 patients with diarrhea produced by enteropathogenic bacteria were examined for leukocytes by MPO activity as well as microscopically using methylene blue stain, MPO activity was positive in 36 patients (92
) and leukocytes were present by microscopic observation in 30 (77
). Fecal leukocytes were not found in healthy controls and the MPO activity was undectable. Stool MPO activity had a range of from 1.6 to 2,830.0 x 10(3) UMPO per gram of feces (median 460.0). The number of neutrophils obtained through MPO activity had a range of 6.0 to 13,216.0/ mm3 (median 1,261.0). Fecal MPO activity is a simple biochemical assay for the detection and quantification of fecal leukocytes.