Determination of orientin,isoorientin,isovitexin in Bamboo Leaf from different sources by HPLC / 中成药

AIM: To determine the contents of orientin,isoorientin,isovitexin in Bamboo Leaf from different sources. METHODS : Orientin,isoorientin,isovitexin were determined by HPLC. The mobile phase was MeOH-H_2O-HAc (35∶65∶1). The detective wavelength was at 340nm. RESULTS : The highest content of three favonoids was 0.89% and the lowest content was 0.07% from different sources. CONCLUSION : This method is simple,quick and accurate for determination of the three favonoids in Bamboo Leaf from different sources.