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Article de Chinois | WPRIM | ID: wpr-690715

RÉSUMÉ

This study aimed to develop an HPLC method for simultaneous determination of the 3 components in Ziziphora bungeana. The optimum HPLC condition was as follows:ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5.0 μm)with gradient elution of methanol (A)-0.2% glacial acetic acid (B), detection wavelength 340 nm,column temperature 30 °C, flow rate 1 mL·min⁻¹. There were good linearity between peak areas and injection quantity of caffeic acid, rosmarinic acid, and linarin in the range of 2-40 mg·L⁻¹(=0.999 9), 3-60 mg·L⁻¹(=1), 7-140 mg·L⁻¹(r=0.999 9), respectively. The average recoveries were 100.9%(RSD 1.3%),98.25%(RSD 2.0%),and 98.73%(RSD 1.5%), respectively. The HPLC method was stable and accurate, which could be used to detect caffeic acid,rosmarinic acid, and linarin in Ziziphora bungeana.

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