Changes of Interleukin-10 and Granulocyte Colony-Stimulating Factor in Cerebrospinal Fluid of Children with Aseptic Meningitis

PURPOSE: Interleukin(IL)-10 is an antiinflammatory cytokine produced by monocytes/macrophages. Granulocyte colony-stimulating factor(G-CSF) plays an important role, not only as a hemopoietic factor but also as a regulating factor for a biologic defense system by neutrophils, in the foci of infection. We studied G-CSF and IL-10 levels in the serum and cerebrospinal fluid(CSF) of patients with aseptic meningitis and investigated the relationship of G-CSF and IL-10 with other inflammatory cells. METHODS: We measured IL-10 and G-CSF levels in the serum and CSF of children with or without aseptic meningitis using ELISA and compared them with other inflammatory cells in the CSF. RESULTS: CSF levels of IL-10 & G-CSF on admission were significantly higher in the aseptic meningitis group than in the control group. IL-10 and G-CSF levels in the CSF were higher than those in the serum(P<0.001). Mean CSF IL-10 & G-CSF levels during the recovery stage decreased significantly compared to those of the symptomatic stage(P<0.001). Significant correlations were found between CSF IL-10 levels and mononuclear cell counts(r=0.26, P<0.05), and between G-CSF levels and neutrophil counts in the CSF(r=0.44, P<0.005). CSF levels of G-CSF were highest on the 1st day of the illness, although CSF IL-10 levels reached its peak on the 3rd day of the illness. CONCLUSION: IL-10 and G-CSF are produced in the CSF of patients with aseptic meningitis and may play an immunoregulatory role by recruiting inflammatory cells from the peripheral blood at the initial stage of aseptic meningitis.

Changes of Interleukin-10 and Granulocyte Colony-Stimulating Factor in Cerebrospinal Fluid of Children with Aseptic Meningitis

PURPOSE: Interleukin(IL)-10 is an antiinflammatory cytokine produced by monocytes/macrophages. Granulocyte colony-stimulating factor(G-CSF) plays an important role, not only as a hemopoietic factor but also as a regulating factor for a biologic defense system by neutrophils, in the foci of infection. We studied G-CSF and IL-10 levels in the serum and cerebrospinal fluid(CSF) of patients with aseptic meningitis and investigated the relationship of G-CSF and IL-10 with other inflammatory cells. METHODS: We measured IL-10 and G-CSF levels in the serum and CSF of children with or without aseptic meningitis using ELISA and compared them with other inflammatory cells in the CSF. RESULTS: CSF levels of IL-10 & G-CSF on admission were significantly higher in the aseptic meningitis group than in the control group. IL-10 and G-CSF levels in the CSF were higher than those in the serum(P<0.001). Mean CSF IL-10 & G-CSF levels during the recovery stage decreased significantly compared to those of the symptomatic stage(P<0.001). Significant correlations were found between CSF IL-10 levels and mononuclear cell counts(r=0.26, P<0.05), and between G-CSF levels and neutrophil counts in the CSF(r=0.44, P<0.005). CSF levels of G-CSF were highest on the 1st day of the illness, although CSF IL-10 levels reached its peak on the 3rd day of the illness. CONCLUSION: IL-10 and G-CSF are produced in the CSF of patients with aseptic meningitis and may play an immunoregulatory role by recruiting inflammatory cells from the peripheral blood at the initial stage of aseptic meningitis.

Primary Lymphoma of Bone Combined with Pancytopenia / 대한혈액학회지

Primary lymphoma of bone, first described by Parker and Jackson in 1939, is derived from bone marrow reticuloendothelial systems. It is uncommon neoplasm representing only 5% of all extranodal non-Hodgkin's lymphoma. Primary bone non-Hodgkin's lymphoma is characterized by initial symptoms of bone pain with associated single or multiple radiological bone lesions, with or without local soft tissue swelling, pathologic fractures,or regional/distant metastasis. Isolated bone marrow non-Hodgkin's lymphoma is rare, and determining the diagnosis difficult. The common features are fever, abnormal liver function, pancytopenia, polyserositis, neurologic symptoms, opportunistic infections, and a short, fatal course. Treatment includes surgery, radiotherapy, and chemotherapy depending on multiple factors, including histopathologic type of tumor, stage of disease, location of the lesion, and extent of osseous involvement. Five year survival rate is above 80%, when treated by combined radiation & chemotherapy. We experienced a patient who admitted to our department of Internal Medicine. She had mutiple bone pain, general weakness and diagnosed as primary lymphoma of the bone with severe pancytopenia. Her presentation, treatment, and clinical progress were discussed.