Seroprevalence of and Identification of Risk Factors in China / 生物医学与环境科学（英文）

Serum samples were tested for IgG antibodies using indirect immunofluorescence assays. We then analyzed associated risk factors. Serum samples were considered positive when reactive at a dilution of more than 1:320. Differences between groups and risk factors associated with exposure were statistically analyzed using Chi-square tests and the generalized linear model. 122 of 1,260 samples (9.68%) were positive for infection. The infection rate ranged from 0% to 30.43% and differed significantly among age groups ( < 0.01); infection rate in the 50-59 years group was significantly higher than that in other age groups. The seroprevalence of varied significantly among sites within the four provinces, and the infection rate of field workers was significantly higher than that of urban workers.

Detecting the concentrations of bisphenol A (BPA) in air of workplaces with HPLC / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To establish the method of detecting the concentrations of bisphenol A (BPA)in air of workplaces with high performance liquid chromatographic (HPLC).</p><p><b>METHODS</b>According to standards of methods for determining the chemical substances in workplace air, BPA in the air was collected by glass fiber filter, then dissolved by acetonitrile and determined by high performance liquid chromatography with FLD.</p><p><b>RESULTS</b>There was a linear relationship within the range of 0.01-10.0 pg /ml, and the detection limit was 0.005 pg/ml. The lowest detected concentration was 3.3x10-5 mg/m3. The relative standard deviation was 2.5-5.5%. The dissolution efficiencies were 95.0%-101.9% and the sampling efficiencies were 99.6%. The samples in glass fiber filter membrane could be stored for 7 days at room temperature.</p><p><b>CONCLUSION</b>The present method could meet with the requirements of Guide for establishing occupational health standards-Part 4 Determination methods of air chemicals in workplace and be feasible for determination of BPA in workplace air.</p>

Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in Changping, Beijing / 中华流行病学杂志

<p><b>OBJECTIVE</b>To detect Bartonella henselae IgG antibody among healthy people in Changping, Beijing.</p><p><b>METHODS</b>Using indirect enzyme linked immunosorbent assay (ELISA) and immunofluorescence antibody assay (IFA) to detect IgG antibody of Bartonella henselae among human beings.</p><p><b>RESULTS</b>The sensitivity and specificity of ELISA were 70.6% and 91.6% respectively, with the positive predictive value of serological test as 82.2%, and the negative predictive value as 84.9%, based on results of IFA. The positive rate was 34.5% among 357 healthy people on indirect ELISA but was 35.6% among 239 people with IFA.</p><p><b>CONCLUSION</b>The results indicated that the indirect ELISA was a very quick, sensitive and available method for detecting Bartonella henselae in human beings, as well as a high positive percent age of Bartonella henselae among the healthy people of Changping Beijing.</p>

Study on Bartonella vinsonii berkhoffii isolated from blood of native dogs in China / 中华流行病学杂志

<p><b>OBJECTIVE</b>To isolate and identify Bartonella strains from native dogs in Shandong province in China.</p><p><b>METHODS</b>EDTA-anticoagulated blood samples were collected from 71 native dogs in Yanggu county of Shandong province in March 2005. All isolates were grown on brain heart infusion agar plates containing 5% defibrinated rabbit blood. The agar plates were incubated at 37 degrees C in a humidified with 5% CO2 environment for 4 weeks or longer. All Bartonella-like isolates were examined by routine Gram and Giménez staining and then followed by polymerase chain reaction (PCR) and PCR-RFLP analysis for identification and differentiation of the isolates. Sequencing 16S rRNA, citrate synthase (gltA) gene and 16S-23S rRNA ITS were carried out and sequential similarities were calculated using the DNASTAR5 software package. The phylogenetic tree was inferred from each bootstrap sample, using the neighbor-joining methods as executed in the MEGA 3.1 software. The translation from DNA to protein were determined by DNASIS 2.5.</p><p><b>RESULTS</b>The two Bartonella-like organisms (strains Q52SHD and Q64SHD) were isolated from the blood of 71 dogs. Light microscopic examination of the Gram and Giménez-stained micro-organisms showed small, short and slightly curved pleomorphic gram-negative bacilli. Amplified products of the three pairs of Bartonella genus-specific primers carried the same size as the predicted of those Bartonella species. Data from PCR-RFLP analysis showed that the two strains that having the same profiles were all different from the B. henselae type strain-16S rRNA, gltA and 16S-23S rRNA ITS sequences from the two isolates were 100.0%, 99.7% and 97.2% homologous to B. vinsonii berkhoffii.</p><p><b>CONCLUSIONS</b>Based on these findings, the two isolates Q52SHD and Q64SHD were demonstrated as B. vinsonii berkhoffii. To our knowledge, this was the first report on the presence of Bartonella infection in native dogs from China, which constituted a large reservoir of Bartonella species in this country.</p>