Résumé
【Objective】 To investigate the characteristics of chronic obstructive pulmonary disease related pulmonary hypertension (COPD-PH) patients and determine the correlation between pulmonary hypertension severity and impaired lung function severity. 【Methods】 A total of 565 patients with COPD who underwent echocardiography and estimated pulmonary artery pressure (sPAP) were recruited. According to whether sPAP was greater than 35 mmHg, the patients were divided into non-COPD-PH group (243 cases) and COPD-PH group (322 cases). According to the level of sPAP, COPD-PH patients were subdivided into mild group, moderate group, and severe group. The degree of airflow limitation in patients with COPD was divided into GOLD 1 to 4. We analyzed the general clinical data characteristics of the patients, pulmonary function indexes differences among patients with different severity of COPD-PH, and the correlation between pulmonary artery pressure and pulmonary function indexes. 【Results】 Compared with patients in the non-COPD-PH group, those in the COPD-PH group were older (P=0.001), had a higher brain natriuretic peptide(BNP) level (P<0.001) and D-dimer level (P=0.020), and a lower diffusing capacity of the lung for carbon monoxide (DLco)% predicted (P=0.001). There were significant differences in the artery partial pressure of oxygen (PaO2) (P=0.017) and DLco% predicted (P=0.004) among patients with mild PH, moderate PH, and severe PH. Severe COPD-PH group had lower PaO2 compared with mild-moderate COPD-PH group, and the higher the PH severity classification, the lower the patients’ DLco% predicted. No significant differences were found in lung ventilation function indexes. There was no significant difference in sPAP among GOLD 1 to 4 groups, and distribution of PH severity and COPD airflow limitation severity was not matched. There was a significant negative correlation between sPAP and DLco% predicted, but no significant correlation between sPAP and lung ventilation indexes. 【Conclusion】 Advanced age, BNP and D-dimer can be used as important predictors for PH occurrence in COPD patients. The degree of hemodynamic impairment does not match the degree of airflow limitation, but it is significantly negatively correlated with diffusion function impairment.
Résumé
The direct acting substances of Cuscuta chinensis in vivo were preliminarily identified through the correlation analysis of "metabolites-effect identification" model. The ovariectomized female rats were i.g administered with 95% ethanol extract part, 40% ethanol elution part and n-butanol extract part of Cuscuta chinensis. The serum fingerprints of different parts and times of administration were established by UPLC/Q-TOF-MS. At the same time, serum estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were detected. Bivariate correlation analysis and grey correlation analysis were used to screen estrogenic components. The results showed that nine direct acting substances in vivo highly related to estrogen effect were found in the drug containing serum, which were hyperoside, astragalin, methyl quercetin glucuronide, quercetin-diglucuronide, quercetin, apigenin, isoquercitrin, kaempferol glucuronide and kaempferol. We can preliminarily screen out the direct acting substance of estrogen effect of Cuscuta chinensis in vivo based on the research idea of serum spectrum effect correlation. It provides a reliable basis for revealing the estrogeneffective substances of Cuscuta chinensis and confirming the quality markers. This experiment was approved by Harbin University of Commerce Ethics Committees (Approval No. HSDU2020-065).
Résumé
Objective To investigate the therapeutical effect of triptolide (TP) on subarachnoid hemorrhage (SAH) in rats and its underlying mechanisms.Methods Endovascular perforation technique was used to establish SAH models.TP was used to treat SAH rats by intraperitoneal injection.Rats were randomly divided into control group,SAH 3 d group,SAH 3 d + DMSO group and SAH 3 d + TP group.Pathologic change was observed in rat cortex by HE staining.Cell apoptosis was evaluated by TUNEL assay.Iba-1 considered as microglia marker was assessed by immunohistochemistry.The levels of inflammatory factors including IL-6,IL-1 β and TNF-o were detected by ELISA.Results Compared with control group,pathologic changes,such as cell edema,nuclear pyknosis were more obviously;the number of apoptosis cell,the expression of Iba-1 and the level of inflammatory factors including IL-6,IL-1β and TNF-α were all increased in the cortex of SAH 3 d group,SAH 3 d + DMSO group and SAH 3 d + TP group (P ＜ 0.05).There were no significant differences in pathologic changes,the number of apoptosis cells,the expression of Iba-1 and the levels of inflammatory cytokines in the brain cortex between SAH 3 d group and SAH 3 d + DMSO group (P ＞ 0.05).Compared with SAH 3 d group and SAH 3 d + DMSO group,pathologic changes were alleviated,and the number of apoptosis cells,the expression of Iba-1 and the levels of inflammatory cytokines including IL-6,IL-1β and TNF-α were all decreased in the brain cortex in SAH 3 d + TP group.Conclusion TP could alleviate the pathologic changes,reduce the cell apoptosis and inhibit the microglia activation in rat brain cortex after SAH,and the neuroprotective effect of TP might be associated with the decreased levels of inflammatory cytokines.