Study On Bioactive Compounds Of Jania Rubens Against Methicillin And Vancomycin Resistant Staphylococcus Aureus

The study is planned to find the antimicrobial activity of the extract of Jania rubens and to isolate the bioactive compound against MRSA and VRSA. Jania rubens collected from Mandapam (Pudumadam) Coastal water, East coast of India and extracted with ethanol. Antibacterial activity of J. rubens was tested against gram positive, gram negative bacteria and drug resistant bacteria). The antibacterial activities were expressed as zone of inhibition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) Identification of compounds from crude extract of J. rubens carried by column chromatography, thin layer chromatography and NMR analysis. Finally J. rubens could serve as useful source of new antibacterial agent.

Laboratory evaluation of bait base for the control of Indian field mouse, Mus booduga (Gray).

Species specific bait evaluation is important for the successful control of rodent populations through rodenticides. Experiments were conducted on the bait preference of Indian field mouse, M. booduga. Results reveal that rice normal grain, rice cracked grain, rice +4% coconut oil and millet +2% coconut oil may be used as effective poison carriers for the Indian field mouse. Additives like oil, sugar and salt did not improve the palatability of bait materials.

Effect of toxaphene toxicity on enzyme activity & residue levels in vital organs of guineapig.

Guineapigs exposed to acute and subacute levels of toxaphene revealed a marginal reduction in the body weight. There was a significant inhibition of acetylcholinesterase and ATPases in the brain, liver and kidney. The effect of subacute toxicity of toxaphene resulted in an enhancement of cytochrome P450 and induction of aniline hydroxylase in liver and kidney. Biochemical investigations on the backbone revealed that toxaphene toxicity caused an increase in the calcium content and a decrease in the collagen content significantly. Toxaphene was accumulated more in the liver than in the kidney as reflected by residue studies.

Effects of dimethoate on collagen metabolism in rats.

The effects of three different doses of dimethoate on the collagen metabolism in the tissues of female albino rats were studied by measuring the specific and total activities of 3H-hydroxyproline in the dermal, gingival and uteral collagen fractions and in the urine. Compared to controls, the total activity of 3H-hydroxyproline in the soluble collagen and in the urine at 12 h after the administration of 3H-proline was significantly lower by 44.45 and 58.12 per cent in the higher dose (2.25 mg/100 g body weight) of dimethoate treated groups respectively. The urinary excretion of hydroxyproline and the total activity of urinary 3H-hydroxyproline measured after 28 days of injection of labelled proline were decreased by 45.56 and 32.68 per cent in higher doses of dimethoate treated animals respectively but the excretions of urinary 3H-hydroxyproline were decreased by 6.36 and 2.88 per cent in lower doses of dimethoate (0.56 mg/100 g body weight) treated animals. The results of the present investigation clearly indicate that the synthesis of collagen is decreased in the higher doses of dimethoate treated animals compared to lower doses of dimethoate treated animals. In addition, the rates of catabolism of both soluble and insoluble collagens were decreased in higher doses of dimethoate treated rats. In concludes that the lower doses of dimethoate (0.56 mg) treated rats were less affected than the higher doses of dimethoate (2.25 mg) treated rats.