Immunolocalization of vascular and non vascular expression of VCAM-1 in hyperplastic, dysplastic oral mucosal lesions and squamous cell carcinoma

38 oral biopsy specimens of hyperplastic surface epithelial lesions [8 cases], epithelial dysplastic lesions [12 cases] and squamous cell carcinoma [SCC] [18 cases] as well as six biopsies of normal oral mucosa were processed for immunohistochemical staining with anti VCAM-1 to identify vascular and non vascular expression of VCAM-1 in the hyperplastic and dysplastic oral lesions as well as oral SCC compared to normal oral mucosa and to examine if it has an active role in the pathogenesis of these lesions that could point the way to the developing of new therapeutic stratigies. The results of this study suggested that VCAM-1 expression has essentially a direct relation with the density and distribution of chronic inflammatory cell infiltration. VCAM-1 is not related to the different grades of epithelial dysplasia and tumor cell differentiation. It cann't be also used as an indicator of tumor angiogenesis

Effect of complete denture on the supporting oral mucosa and alveolar bone in-patients with hypohidrotic ectodermal dysplasia [HED]

Four young male children [10-13 years] referred from dermatology clinic for prosthetic management .The children were diagnosed as hypohidrotic ectodermal dysplasia with complete an-odontia of their deciduous and permanent dentition. Full upper and lower acrylic dentures were constructed for those children for physiologic and psychosocial reasons .Cytological and radiographical evaluations were carried out before and after denture wearing. Cytological examination before denture insertion showed the presence of large prickly and rounded epithelial cells indicating either incomplete differentiation of the covering surface epithelium, that is normally keratinized. After denture insertion the predominant exfoliated cells were found to be spindleform flattened cells. This may reflect the protective effect of denture wearing on the integrity, of the surface epithelium. Radiographic examinations showing slight increase in the amount of alveolar bone loss after denture insertion

effect of demineralized bone powder on the healing of irradiated bone deffects post-operatively in the mandible of rats

The contribution of the tissue components in the healing of bony defects after irradiation was studied using demineralized bone powder [DBP] prepared. In this study 18 rats were divided into two groups, nine rats each. The second group was irradiated after three weeks post-irradiation. The operation was done with two cavities, one filled with DBP and the other one was left empty. The second group was operated without irradiation, then after two weeks from the surgery, three rats were killed, and the same thing was done after four weeks and eight weeks. The specimens were fixed, decalcified and processed routinely for H and E. It was found that the unirradiated bony defect with DBP showed the best healing involving the quantity and quality of bone filled defects, followed by the unirradiated bony defect without DBP, then the irradiated bone defect with DBP, and lastly, the irradiated bone defect without DBP that had the least quality of new bone

Immunohistochemical detection of keratin proteins in the surface epithelium adjacent to oral squamous cell carcinoma

The surface epithelium adjacent to 32 cases of oral squamous cell carcinoma were taken, fixed, processed and stained with routine H and E and antikeratin antibody [Dako Pat Kit K 522]. The surface epithelium adjacent to oral squamous cell carcinoma showed variable changes as normal hyperplastic, dysplastic epithelium including areas with leukoplakia and carcinoma in situ. The expression of keratin proteins in normal, hyperplastic and mild dysplastic surface epithelium showed a regular and regional distribution pattern. On the other hand, the whole thickness of the surface epithelium with moderate and severe dysplastic changes involving areas with leukoplakia and carcinoma in situ exhibited irregular immunostaining pattern where variable numbers of the basal and superbasal cells displayed positive reaction. The reaction lead to the suggestion that there was a correlation between the pattern of keratin distribution the degree of cellular differention as well as this marker can be considered as a helpful aid in the diagnosis of moderate and severe dysplasia including carcinoma in situ especially in small fragmented biopsies. Based on the staining intensity, the cell of leukoplakia can be also considered as highly differentiated cells whereas that of carcinoma in situ can be considered as less differentiated cells