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Zhongguo Zhong Yao Za Zhi ; (24): 1818-1821, 2006.
Article de Chinois | WPRIM | ID: wpr-315948

RÉSUMÉ

<p><b>OBJECTIVE</b>To elucidate the cytotoxicity and mechanism of 23-O-acetylcimigenol-3-O-beta-D-xylopyranoside isolated from C. dahurica on HepG2 cells and to find the leading compound for new drug development.</p><p><b>METHOD</b>MTT, AO/EB staining observation, flow cytometry and western blot methods were used to study the cytotoxicity, morphological changes, cell cycle distribution and protein expression profile of 23-O-acetylcimigenol-3-O-beta-D-xylopyranoside on HepG2 cells.</p><p><b>RESULT</b>23-O-acetylcimigenol-3-O-beta-D-xylopyranoside could inhibit the proliferation of HepG2 cells with IC50 at 16 micromol x L(-1), and could also induce apoptosis and G2-M cell cycle arrest. Further study demonstrated that the compound could cleavage PARP, regulate protein expression of bcl-2 family and decrease the expression of cdc 2 and cyclin B.</p><p><b>CONCLUSION</b>23-O-acetylcimigenol-3-O-beta-D-xylopyranoside exerts its cytotoxicity on HepG2 cells via apoptosis and G2-M arrest. In addition, caspases family activation, regulation of protein expression of bcl-2 family and down regulation of cdc 2 and cyclin B were involved in apoptosis and G2-M arrest induced by it.</p>


Sujet(s)
Humains , Apoptose , Protéine-kinase CDC2 , Métabolisme , Cycle cellulaire , Lignée cellulaire tumorale , Prolifération cellulaire , Cimicifuga , Chimie , Cycline B , Métabolisme , Hétérosides , Pharmacologie , Tumeurs du foie , Métabolisme , Anatomopathologie , Plantes médicinales , Chimie , Poly(ADP-ribose) polymerases , Métabolisme , Protéines proto-oncogènes c-bcl-2 , Métabolisme , Triterpènes , Pharmacologie , Protéine Bax , Métabolisme
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