Résumé
8-Methoxypsoralen [8-MOP] is a photoactive compound widely used in the treatment of proliferate disorders. The present study investigates the effects of 8-MOP on ovary function and pituitary-gonad axis in mice. In this experimental analytical study, 45 female Balb/C mice were divided into three groups [n=15], control, sham [olive oil injection] and experimental. The experimental group were received an intraperitoneal [i.p.] injection of the LD50 dose of 60 mg/kg 8-MOP. At 30 days after injection, the animals were sacrificed while in the proestrus stage and examined for morphological and histological changes their ovaries. Blood samples were collected and estrogen, luteinizing hormone [LH] and follicle stimulating hormone [FSH] levels were assessed by radioimmunoassay. Data were analyzed using one-way ANOVA and the t test. The mean levels of estrogen and progesterone in the experimental group significantly decreased [p<0.001]. However, there was a significant increase in LH and FSH levels in this group compared to the control groups [p<0.001]. The mean number and diameter of the corpus luteum [CL] and the number of growing follicles in the experimental group significantly reduced compared to the control and sham groups [p<0.001]. The mean granulosa thickness in the experimental group also significantly decreased compared to the control and sham groups [p<0.001]. Our data indicated that 8-MOP can affect the levels of LH, FSH, estrogen and progesterone. Our findings further suggest that consecutive doses of 8-MOP may impair the female reproductive tract [or development]
Résumé
Ecstasy [methylendioxymethamphetamine] is a stimulant drug that has various side effects on nerve system, cardiovascular and immune system. This substance affects body tissues and can cause their death. Therefore, in the present study, we investigated the effects of ecstasy on liver tissues in Wistar rats. Fifty Wistar rats, ranging 5-6 weeks of age, were divided into five groups [n=10] including control, sham, experimental 1, 2 and 3. In the experimental groups, the animals were injected with the consecutive doses of ecstasy [2, 4 and 8 mg/kg, I.P, for two weeks]. The sham group received normal saline and the control group had no injection. Animals were sacrificed 12 hours after the latest injection of ecstasy. Liver tissues sections were provided and stained by hematoxylin-eosin to investigate the histopathological changes. Hepatocytes cells significantly decreased in experiment groups as compared with the control and sham groups [p<0.05]. This decrease was more severe with higher doses. The results showed that numbers of kuppfer cells and liver weight were increased and the degree of changes depended on the dose. According to the results, ecstasy might cause damages on liver tissue in higher and consecutive doses. So, this study suggests more attention on using of these kinds of drugs