Usage of computational software for analysis of human cytomegalovirus specific primers

Basic Local Alignment Search Tool [BLAST] is important software for molecular biologists. We suggested BLAST usage to test HCMV specific primers reliability before being used in diagnostic PCR. 158 HCMV primers were extracted from PubMed articles, and then tested by BLAST against nucleotide sequences database of HCMV and other organisms. These studied primers showed mismatch with all or some HCMV nucleotide sequences; or perfect matching with all HCMV nucleotide sequences, or some nucleotide sequences of other organisms. Using BLAST to test primers could be useful to investigate their specificity, sequences accuracy, ability to detect all HCMV strains, and finally selected genetic target regions

Using of indirect Immuno florescence[IIF]method and enzyme-linked Immuno sorbant assay[ELISA]method in detecting of spermatozoa antibodies in serum

We proposed to compare between Indirect Immuno Fluorescence [IIF] method and Enzyme-Linked Immuno Sorbant Assay [ELISA] method in detecting spermatozoa antibodies in serum, and also to study the relationship between the presence of antisperm antibodies in serum and unexplained infertility. Fifty-four patients aged 18-45 years [30 men and 24 women] suffered from unexplained infertility [all their routine tests for evaluating infertility are normal] were evaluated by detecting spermatozoa antibodies in their serum by two methods [IIF and ELISA]. On the other hand, forty-five fertile men aged 16- 50 years were evaluated in the same way as controls [who conceived last child two years or less before the study]. Out of fifty-four patients, twenty-two patient's serum [11 men and 11 women] were positive for antisperm antibodies by using IIF method. Three serums of the control men were also positive. Whereas, twenty patient's serum [12 men and 8 women] and four control's serum were positive for Antisperm antibodies by using ELISA method. Our study revealed that there was a considerable correlation between the presence of serum-antisperm antibodies and unexplained infertility [P = 0.000102]. This finding highlights the role of antisperm antibodies in unexplained infertility. Moreover, we found that ELISA method had low sensitivity [32%] and specificity [78,3%] when compared with IIF method in detecting spermatozoa antibodies in serum. This finding suggests that IIF method is the method of choice for evaluating antisperm antibodies in serum