[Introduction and bioinformatic's evaluation of candidate genes in breast cancer using cytogenetic studies of cancer induction]

Discovery of genetic changes which contribute to cellular neoplastic and malignant tumor transformation is one of the major aims in oncology researches. The aim of this study was to investigate the DMBA-induced breast cancer in SD rat strains using bioinformatical methods and also to find their homologous regions in human chromosomes. In this research, we used SD rat strains as a suitable model for DMBA-induced breast cancer. We gavaged the rats twice with 10 mg DMBA solved in 0.5 ml sesame oil. After tumors appeared in DMBA-treated rats, they were subjected to histopathology and immunohistochemistery studies, cell culture, metaphase chromosomal preparation, and finally G-banding stain. According to databases, we collected genes in the affected area and prepared a gene list by comparing genome of the rats and human in changed chromosomal segments. Our data showed numerical and frequent structural changes in different number of chromosomes. For example, we found recurrent gain in chromosomes 3, 4, 8, 12, 17, loss in chromosomes 3, 9, 12 and 15, also deletion in chromosomes 2, 3, 4, 6, 7, 20 and addition in chromosomes 11, 15 and 19. According to these chromosomal changes and based on bioinformatics studies we predict that the gene TGFBR3, HACE1, UBR5, CALB2, HPR, LCP1, RRM2B, ABO, ZFHX3, TNFSF11, ABL1, EPSTI1, PRDM1, REG3A, FOXA1 and PRKD1, probably may contribute to the development of breast cancer

[Plasma free 8-iso-PGF2alpha levels in normotensive and preeclamptic pregnancies]

Preeclampsia is a main cause of morbidity and mortality of pregnant women worldwide. Recently oxidative stress was considered as a part of pathophysiolgy of preeclamsia and 8-isc% prostaglandin F2alpha [8-iso-PGF2 alpha] is known as a marker of oxidative stress. The purpose of this study was to measure and compare plasma concentration of free 8-iso-PG F2 alpha in women with normotensive and preeclamptic pregnancies. In this descriptive-analytic study, Plasma free 8-iso-PGF2 alpha levels were measured in pregnant women with preeclamsia [n=42] and normotensive pregnant women [n=39] between 28-38 weeks of gestation. Women in two groups were matched in age, gestational age and socioeconomic statue and all women were nulligravid. Free 8-iso-PGF2 alpha levels were not significantly different in women with preeclamsia and normotensive group [17808 vs. 24288 pg/dl, NS]. Plasma free 8-iso-PGF2 alpha levels were increased in severe compared to mild preeclampsia [NS]. Neonates weight of preeclamptic mothers was significantly lower than neonates of normotensive women [p<0.0001]. Although free 8-iso-PGF2 alpha is a marker of oxidative stress in vitro, but its measurement is not valuable in clinical medicine. However, further studies in normal pregnancy and preeclampsia will be needed