Establishment of autochthonous and standard cell line derived xenograft models of glioblastoma multiform in Iran

GBM is the most common and malignant astrocity tumor and it is persistent to common treatment so, these patients have a very low survival. Several researchers around the world, including Iran, have been investigated GBM-cell line in vitro. However in vivo studies have not been fulfilled. As standard cell line [U-87MG] derived from human GBM and total GBM tumor derived from 3 patients were heterotypic ally injected into 4-6 weeks old athymic nude mice. Pathologic investigation by H and E, GFAP and Ki-67 were examined 2 months post implantation. GBM characteristics appeared in H and E and GFAP and the rate of proliferation was 6% and in direct xenograft tumor was 9% which was consistent with the pathologic result of patient. GBM Xengraft is the most suitable model for in vivo investigation and researcher can evaluate new treatments for this tumor. On the other hands, Pharmacogenomics differences in treatment response could be indicated among Iranians

[Effect of gamma irradiation sterilization on the osteoinductive capacity of demineralized bone matrix]

Gamma irradiation has been widely used for secondary sterilization of bone allograft before clinical use to reduce the risk of infection and associated complications. The current study evaluated the effect of gamma irradiation sterilization on the osteoinductive capability of human demineralized bone matrix using a rat model. Eighteen euthymic rats received two separate implants consisting of 30 mg sterile-harvest demineralized bone matrix and 30 mg gamma irradiation-sterilized demineralized bone matrix. The demineralized bone matrix from each group was placed into two separate muscle pouches created in the paravertebral muscles of each rat. All 18 rats were euthanized after 4 weeks and each implantation site was removed with 0, 5 cm normal tissue around the implant. Histological examination was performed to determine the presence or absence of new bone, cartilage and bone marrow elements, All except one of 18 [94.4%] sterile-harvest demineralized bone matrix sites histologically contained new bone elements and five of 18 [27.7%] ethylene oxide sterilized demineralized bone matrix sites showed evidence of new bone elements, which was statistically significant [p<0.05]. The results of this study indicate that demineralized bone matrix sterilized with gamma irradiation loses its osteoinductive capacity in a manner similar to that of steamsterilized demineralized bone matrix, making it unsuitable as a method of secondary sterilization of demineralized bone matrix

Immunohistochemical analysis of estrogen and progesterone receptor expression in gingival lesions

Some lesions in the oral cavity and mostly on gingiva have predominant predilection towards females, and mostly occur in the first four decades of life when changes in sex hormone levels in blood are obvious. The present study aimed to investigate the presence and distribution of estrogen and progesterone receptors in peripheral giant cell granuloma [PGCG], pyogenic granuloma [PG] and peripheral ossifying fibroma [POF] on gingiva as an organ target. In a descriptive case series study from March 2002 to April 2003, paraffin blocks from patients with exophitic lesion on gingiva, diagnosed by histopathology as PGCG, PG or POF at Dentistry Faculty of Tehran University of Medical Sciences [TUMS], Iran, were analyzed with Immunohistochemical [IHC] technique. The data analysis was performed by frequency and descriptive statistics. Of 35 patients, 12 estrogen receptors [ERS] and progesterone receptors [PRS] were detected. Nine of them were PRS and three were ERS. Two third of ERS/ PRS were seen in females and one third in males, respectively. In order of decreasing frequency the ERS and PRS were found in PG [n=6], POF [n=4] and PGCG [n=2]. In this study, ER/ PR were revealed in three lesions. PR was detected in all of three lesions but we could not see ER in PGCG. Thus, gingiva may be considered as a target organ for sex hormones