Monosaccharide composition analysis and its content determination of polysaccharides from Rhaponticum uniforum / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze the monosaccharide composition in the polysaccharides from Rhaponticum uniforum, determine the content of monosaccharide, and provide some references for further research.</p><p><b>METHOD</b>The monosaccharide composition was determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Phenol-sulfuric acid method was used for the determination of the content of polysaccharide.</p><p><b>RESULT</b>The monosaccharides composition in polysaccharides from R. uniforum are glucose, arabonose and fructose. Their molar ratios are 1 : 1.61 : 2.21. The content of polysaccharide is 95.78%, taking the mixture of monosaccharide compositions as reference substances.</p><p><b>CONCLUSION</b>HPAEC-PAD can be used to analyze the monosaccharide composition in the polysaccharide with high precision, and the method of phenol-sulfuric acid is simple, convenient and reliable.</p>

Effects of Rhaponticum uniforum polysaccharide on immune response of mice after antigen stimulation and their possible mechanisms / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effects of Rhaponticum uniforum polysaccharide on immune function in normal mice and the underlying mechanism.</p><p><b>METHOD</b>SRBC and ovalbumin were employed as antigens to be injected to mice, respectively. Three doses of R. uniforum polysaccharide (50, 100, 200 mg x kg(-1) x d(-1)) were given orally for seven days. After the secondary immunization, the level of corresponding antibody and the concentration of serum IL-2, IFN-gamma were determined.</p><p><b>RESULT</b>The levels of antibodies (anti-SRBC and anti-ovalbumin) and cytokines (IL-2 and IFN-gamma) in median dose group of R. uniforum polysaccharide were all significantly higher than those in control groups (P <0.05).</p><p><b>CONCLUSION</b>R. uniforum polysaccharide could enhance the immune function in normal mice.</p>