Co-culture of Mouse Two-cell Embryos on Human Endometrial Stromal Cells in Proliferative and Secretory Phases / Co-cultivo de Dos Células Embrionarias de Ratón sobre Células Estromales Endometriales Humanos en Fases Proliferativa y Secretora

There were no significant differences in the distribution of embryos reaching to 2- cells, 4- cells, morula or blastocysts culturing on human endometrial stromal cells (Secretory or proliferative phases). The percent of morula in stage A (without fragmentation), stage B (<25% fragmentation), stage C (25-50% fragmentation) and stage D (>50% fragmentation) and did not showed significant differences between two coculture groups. Thus, the phase that the endometrial stromal cells were in thereby did not affect on the quality of embryos.

No hubo diferencias significativas en la distribución de los embriones en los cultivos que llegan a las 2 y 4 células, mórula o blastocistos sobre las células del estroma endometrial (fases proliferativa y secretora). El porcentaje de mórulas en etapa A (sin fragmentación), etapa B (<25% fragmentación), etapa C (25-50% de fragmentación) y etapa D (>50% fragmentación), y no mostraron diferencias significativas entre los dos grupos de co-cultivo. Así, la fase en la que se encontraban las células estromales endometriales no afectaron la calidad de los embriones.

comparison of anastrozole and clomiphene citrate for ovulation induction in women with unexplained infertility undergoing intrauterine insemination

In order to overcome the undesirable effects of clomiphene citrate [CC] alternative regimens such as Aromatase inhibitors [AIs] have been recommended. Our objective was to compare the ovarian and endometrial effects of three day Anastrozole and clomiphene citrate in combination with gonadotropin in IUI cycles. This was a prospective descriptive study in 55 infertile patients with unexplained infertility. The patients in Group A [29 patients] received CC and gonadotropin, in contrast to group B [26 patients] who received Anastrozole and gonadotropin. Primary outcome was the size and number of mature follicles together with endometrial thickness. On the day of HCG administration Anastrozole group developed fewer mature follicles [0.54 +/- 0.64 vs. 1.07 +/- 1.06 P=0.032] and fewer total mature follicles [17 vs 31] compared with CC group, however the endometrial stripe thickness were similar in both group [7.73 +/- 2.54 vs. 7.89 +/- 1.93 P=0.800] Three day Anastrozole in addition to gonadotropin in IUI cycles develop fewer mature follicles significantly at the time of HCG- injection than those in C.C group, in unexplained infertility in IUI cycles. This dosage is equivalent to traditional CC dosage in IUI cycles on endometrial development