Résumé
Objective: Mesenchymal stem cells [MSCs], due to their immunomodulatory functions, are an ideal candidate for the treatment of immune-related diseases. Recurrent spontaneous abortion [RSA] is one of the most common complications of pregnancy which in many cases is related to the immune system disorders. Our previous study has shown that the abortion rate was decreased following the syngeneic MSCs therapy in abortion-prone mice. In this study, the therapeutic effect of syngeneic, allogeneic, and xenogeneic MSCs was compared in a mouse model of RSA
Materials and Methods: In this experimental study, MSCs were isolated from adipose tissue [ASCs] of CBA/J and BALB/c mice and human. After characterization, ASCs were injected [IP] at day 4 of gestation to female CBA/J mice following their mating with DBA/2 male mice. In the control group, phosphate-buffered saline [PBS] was injected and CBA/J×BALB/c mating was also used as the normal pregnancy control. On day 14.5 of pregnancy, embryo resorption rate was determined
Results: The abortion rate significantly decreased following the ASCs therapy from syngeneic [6.31%], allogeneic [6.54%], and xenogeneic group [12.36%] compared to ASCs non-treated group [34.4%]. There was no statistical difference between ASCs treated groups, however syngeneic and allogeneic ASCs reduced the abortion rate more efficiently than xenogeneic ASC
Conclusion: The abortion rate was significantly decreased following the intraperitoneal administration of ASCs from various donated sources in abortion-prone mice. These results indicated that the immunogenicity of allogeneic and xenogeneic ASCs is not a contradictory problem for their therapeutic effects on RSA
Résumé
Background: Detection and quantification of human Papillomavirus [HPV] genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease
Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma
Results: Eighteen [36%] specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 [55.55%], and 8 specimens were positive for HPV-11 [44.44%]. Of the 18 infected specimens, 6 [33.32%] and 12 [66.65%] were identified as high-titer and low-titer viral load, respectively
Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies
Résumé
Hydatid cyst is one of the parasitic zoonotic diseases in Iran and the world, and a significant percentage of hospital beds are annually allocated to these patients. This study aimed to determine the epidemiological status of hydatid cyst in hospitals affiliated to Qom University of Medical Sciences during a 12-year period. In this descriptive-analytic study, the medical records of all patients with hydatid cyst who underwent surgery in hospitals of Qom University of Medical Sciences from 2002 to 2013, were investigated. The demographical and clinical data were extracted from patients' records. Data analysis was performed using chi square test. The number of patients during this period was 82. The mean age of patients was 38.80 +/- 17.20 [min=8 and max=69]. 57.3% of the patients were female and 42.7% were male. The highest percentage of hydatid cyst was seen in menin the age group of 31-40 years [37.15%], and in women, in the age group of 21-31 [25.53%] were in the age group 21-30 years old. Frequency of the disease among housewives [51.3%] was higher than other occupational groups. Most of the patients [90%] lived in urban areas. Liver infection was seen in 74.3% of the patients. Considering that the highest frequency of the disease was reported in economically active age group and that housewives are more at risk for this disease, therefore training programs for the prevention and control of the disease appears to be essential for target groups
Résumé
Aspirin is the drug of the century, and is a multifunctional drug and one of the most prescribed drugs in the world. Aspirin is a safe drug at low doses but also it has life-threatening side effects when administered at high doses. This study investigates the effects of aspirin on renal cortical and medullary tissue in rat embryos. In this study, 30 pregnant female rats were randomly divided into 6 groups. Control group with no intervention, sham group received 2 ml distilled water [as a solvent of aspirin] received from days 8 to 20 of pregnancy, and four experimental groups received different doses of 75, 100, 200 and 300 mg/kg of aspirin by gavage. Pregnant rats were sacrificed on the twenty days of pregnancy and the fetuses were removed. Weight of the fetuses and placenta and Crown-Rump length were measured. Fetal kidneys were fixed in formalin processed, sectioned and stained with Hematoxylin-Eosin. Thickness of renal cortical and medullary tissue by using a Motic hardware and software system were measured and recorded. A significance level of 0.05 was predetermined for all statistical analyses. No apparent fetal anomalies were observed in experimental groups. In addition, no significant differences were shown in the mean of fetal weight, placental weight, mean of Crown-Rump length in experimental groups 75, 200 and 300 mg/kg compared to control and sham groups. Mean fetal and placental weight in experimental group 100 significantly increased compared to control and sham groups. Mean ratio of renal cortex to renal medulla in experimental group 75, 100 and 300 were significantly decreased compared to control and sham groups [respectively P = 0.03, P = 0.013, P = 0.03]. It seems that maternal use of aspirin during pregnancy can not cause fetal abnormalities. However, it can cause some changes in renal cortical and medullary tissue of rat embryos