Résumé
Objective:To explore influence of secondary turntable abdomen insulin injection positioning ruler (STAI-IPR)on blood glucose level and complications in aged patients with diabetes mellitus (DM).Methods:A total of 120 aged DM patients treated in our hospital from Jan 2016 to Dec 2016 were selected.According to random number ta-ble,patients were randomly and equally divided into random rotation group (received random rotated injection)and STAIIPR group (received positioning injection by STAIIPR),both groups were injected for 1000 times.Levels of fasting blood glucose (FBG)and 2h postprandial blood glucose (2hPG)before and after treatment,pain score and incidence of complications after treatment were observed and compared between two groups.Results:Compared with before treatment,there were significant reductions in levels of FBG and 2hPG in both groups after treatment, P =0.001 all.Compared with random rotation group after treatment,there were significant reductions in levels of FBG [(6.58±1.07)mmol/L vs.(5.67±1.58)mmol/L],2hPG [(9.35±1.59)mmol/L vs.(8.41±1.27)mmol/L]and incidence rate of complications (20.0% vs.5.0%);and significant rise in percentage of 0 pain score (30.0% vs.55.0%)in STAIIPR group,P <0.05 or <0.01. Conclusion:Insulin injection by STAIIPR can disperse injection point without overlap.It can effectively control blood glucose level,reduce complications of injection sites and is easy for patients to do it themselves in aged DM patients,which is worth clinical extension.
Résumé
Objective:To explore influence of secondary turntable abdomen insulin injection positioning ruler (STAI-IPR)on blood glucose level and complications in aged patients with diabetes mellitus (DM).Methods:A total of 120 aged DM patients treated in our hospital from Jan 2016 to Dec 2016 were selected.According to random number ta-ble,patients were randomly and equally divided into random rotation group (received random rotated injection)and STAIIPR group (received positioning injection by STAIIPR),both groups were injected for 1000 times.Levels of fasting blood glucose (FBG)and 2h postprandial blood glucose (2hPG)before and after treatment,pain score and incidence of complications after treatment were observed and compared between two groups.Results:Compared with before treatment,there were significant reductions in levels of FBG and 2hPG in both groups after treatment, P =0.001 all.Compared with random rotation group after treatment,there were significant reductions in levels of FBG [(6.58±1.07)mmol/L vs.(5.67±1.58)mmol/L],2hPG [(9.35±1.59)mmol/L vs.(8.41±1.27)mmol/L]and incidence rate of complications (20.0% vs.5.0%);and significant rise in percentage of 0 pain score (30.0% vs.55.0%)in STAIIPR group,P <0.05 or <0.01. Conclusion:Insulin injection by STAIIPR can disperse injection point without overlap.It can effectively control blood glucose level,reduce complications of injection sites and is easy for patients to do it themselves in aged DM patients,which is worth clinical extension.
Résumé
In this report, we developed a HBV infection model in C57BL/6 mouse line by in vivo injection of a recombinant adeno-associated virus 8 vector carrying 1. 3 copies of HBV genome (ayw subtype) (rAAV8-1. 3HBV). We firstly prepared and purified the rAAV8-1. 3HBV and then injected it into three C57BL/6 mice with the dose of 2 x 10e11vg, respectively. HBsAg and HBeAg were assayed in sera collected at different time points post injection. Ten weeks post injection, the three mice were sacrificed and blood and liver tissue were taken for assay. Copies of HBV DNA were detected by real time PCR and the way of HBV DNA replication was identified by PCR. Subsequently, detection of HBV antigen by immunohistochemistry and pathology analysis of liver tissue of mice were performed. The results suggested that expression of HBsAg and HBeAg lasted for at least 10 weeks in mice sera. Among mice injected with rAAV8-1. 3HBV, HBsAg levels were showed an 'increasing-decreasing-increasing' pattern (the lowest level at the 4th week post injection), while HBeAg levels were kept high and relatively stable. HBV DNA copies were 4.2 x 10(3), 3.6 x 10(3), 2.5 x 10(3) copies/mL in sera and 8.0 x 10(6), 5.7 x 10(6), 2.6 x 10(6) copies/g in hepatic tissues of three mice, respectively. We found that the linear 1. 3HBV DNA in the rAAV8-1. 3HBV could self form into circular HBV genome and replicate in livers of HBV transfected mice. HBsAg and HBcAg were both positive in liver tissue of mice injected with rAAV8-1. 3HBV and no obvious pathological characters were found in liver of mice injected with rAAV8-1. 3HBV. In conclusion, we successfully developed a HBV chronic infection model in C57BL/6 mouse line by in vivo transduction with the recombinant virus rAAV8-1. 3HBV, in which HBV genes could be continuously expressed and replicated over 10 weeks, and paved a way for further characterization of the human chronic hepatitis B virus infection and evaluation of vaccine and anti-HBV agents.