Antibodies to citrullinated peptides are not associated with the rate of joint destruction in patients with a well-established diagnosis of rheumatoid arthritis

Antibodies to citrullinated peptides are highly specific for rheumatoid arthritis (RA) and represent a significant risk factor for undifferentiated polyarthritis. This prognostic ability may be related to the very diagnostic performance of these autoantibodies, since RA is a more erosive disease than other forms of arthritis. The present study evaluated an association of antibodies to citrullinated peptides and the rate of joint destruction in patients with a well-established diagnosis of RA. Seventy-one patients with RA were evaluated in 1994 and again in 2002 (functional class, joint count, Health Assessment Questionnaire score, hands X-ray). Autoantibodies (rheumatoid factor (RF), anti-perinuclear factor, anti-cyclic citrullinated peptide (CCP) antibodies) and Sharp's index were analyzed blindly. Delta Sharp was calculated as the difference in Sharp's index obtained in 1994 and 2002. During the follow-up the Health Assessment Questionnaire score increased from 0.91 ± 0.74 to 1.39 ± 0.72 (P < 0.001). Similarly, the number of swollen joints increased from 4.6 ± 5.71 to 6.4 ± 4.1 (P = 0.002). The frequency of autoantibodies and anti-CCP titer remained stable; however, serum RF concentration increased from 202.8 ± 357.6 to 416.6 ± 636.5 IU/mL (P = 0.003). Sharp's index increased from 56.7 ± 62.1 to 92.4 ± 80.9 (P < 0.001). No correlation was observed between Delta Sharp and the presence of RF, anti-perinuclear factor, and anti-CCP antibodies at baseline. Antibodies to citrullinated epitopes are specific and early markers for the diagnosis of RA but do not seem to be associated with the rate of joint destruction in patients with a well-established diagnosis of RA.

Características da carcaça e da carne de bovinos sob diferentes dietas, em confinamento / Carcass and meat characteristic of cattle receiving differents diets in feedlot

Avaliaram-se as características qualitativas da carcaça e da carne de 10 tourinhos, de 10 machos castrados e de 10 fêmeas da raça Canchim, terminados em confinamento e alimentados com: 1 - silagem de milho e concentrado contendo farelo de soja, milho e polpa cítrica (SM) e 2 - cana-de-açúcar e concentrado contendo farelo de soja, milho, grãos de girassol e polpa cítrica (CA). O período experimental foi de 105 dias, e o delineamento foi o inteiramente ao acaso, em esquema fatorial 3 x 2 (3 condições sexuais e 2 dietas). Não foram observadas interações e os tourinhos apresentaram maiores (P<0,05) peso da carcaça quente e da área de olho de lombo, maior rendimento de dianteiro e menor rendimento de traseiro em relação aos machos castrados e às fêmeas. Nas amostras de contrafilé, não houve diferença entre tratamentos quanto à força de cisalhamento, pH, perdas totais por gotejamento e capacidade de retenção de água e cor da carne. Os machos castrados e as fêmeas apresentaram carne de melhor textura. As dietas SM e CA podem ser utilizadas na terminação de bovinos em confinamento.

The carcass and meat qualitative characteristics of 10 young bulls, 10 castrated males, and 10 females of Canchim breed finished in feedlot were evaluated. Animals were fed: 1 - corn silage and concentrate containing soybean meal, corn and citrus pulp (CS), and 2 - sugar cane and concentrate containing soybean meal, corn, sunflower grains, and citrus pulp (SC). The data were analized by completely randomized design in a factorial 3x2 arrangement (three sexual conditions x two diets), and the experimental period lasted 105 days. No interactions were observed and the young bulls presented higher (P<0.05) hot carcass weights and loin area, higher hindquarter and lower forequarter yields in relation to the castrated males and females. The samples of loin did not show differences among treatments in relation to shear force, pH, total losses due dripping on capacity of water retention, and meat color. The castrated males and females presented better texture of meat. The CS and SC diets can be used in finishing cattle under confinement.

Utilization of different methodologies for the characterization of Hb Hasharon heterozygotes

Hb Hasharon has an electrophoretic mobility similar to that of Hb S in cellulose acetate and a mobility between Hb S and C at acid pH. In high-performance liquid chromatography, Hb Hasharon shows a distinct chromatographic profile and retention time. The origin of this variant is a mutation in codon 47 (GAC --> CAC) of the alpha2-globin gene, resulting in the replacement of asparagine by histidine during the translation process. Ten blood samples from individuals suspected of being Hb Hasharon carriers were analyzed. In addition to classic laboratory tests and high-performance liquid chromatography, molecular analysis by polymerase chain reaction with restriction fragment length polymorphism designed in the laboratory was performed to confirm this mutation. The study of these cases showed that a combination of classical and molecular methodologies is necessary in the diagnosis of hemoglobinopathies for a correct hemoglobin mutant identification. The accurate identification of hemoglobin variants is essential for genetic counseling and choice of therapy.

Reduced bone mineral density in men after heart transplantation

Heart transplantation is associated with rapid bone loss and an increased prevalence and incidence of fractures. The aim of the present study was to compare the bone mineral density (BMD) of 30 heart transplant (HT) recipients to that of 31 chronic heart failure (CHF) patients waiting for transplantation and to determine their biochemical markers of bone resorption and hormone levels. The BMD of lumbar spine and proximal femur was determined by dual-energy X-ray absorptiometry. Anteroposterior and lateral radiographs of the thoracic and lumbar spine were also obtained. The mean age of the two groups did not differ significantly. Mean time of transplantation was 25.4 + ou - 21.1 months (6 to 88 months). Except for the albumin levels, which were significantly higher, and magnesium levels, which were significantly lower in HT patients when compared to CHF patients, all other biochemical parameters and hormone levels were within the normal range and similar in the two groups. Both groups had lower BMD of the spine and proximal femur compared to young healthy adults. However, the mean BMD of HT patients was significantly lower than in CHF patients at all sites studied. Bone mass did not correlate with time after transplantation or cumulative dose of cyclosporine A. There was a negative correlation between BMD and the cumulative dose of prednisone. These data suggest that bone loss occurs in HT patients mainly due to the use of corticosteroids and that in 30 per cent of the patients it can be present before transplantation. It seems that cyclosporine A may also play a role in this loss.

Sarcoidose - relato de um caso salientando o comprometimento osseo. / Sarcoidosis: report of a case with bone involvement

Os autores relatam um caso de sarcoidose com acometimento osseo, salientando o estudo radiologico das lesoes osseas