Effects of tetrandrine on pulmonary capillary permeability in rats with acute lung injury induced by lipopolysaccharide / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the effects of tetrandrine (Tet) on the pulmonary capillary permeability in rats with acute lung injury induced by intravenous injection of lipopolysaccharide (LPS).</p><p><b>METHODS</b>Thirty-two healthy male Wistar rats were randomly divided into 4 groups: the model group, the normal group, the Tet prevention group and the Tet treatment group, 8 in each group. All rats except those in the normal group, were established into acute lung injury model by intravenous injection of LPS. Intravenous injection of Tet (20 mg/ kg) were given to the Tet prevention group 30 min before and to the Tet treatment group 30 min after modeling respectively, and equal volume of normal saline was given to the other two groups instead. Arterial blood samples were collected to determine PaO2 and PaCO2 at the time points of immediately after modeling (0 h), 0.5 h, 2 h, 4 h, 6 h. The animals were sacrificed by the end of the experiment, the wet/dry weight ratio of lung (W/ D), neutrophil percentage in bronchoalveolar lavage fluid (BALF), concentration of TNF-a in peripheral blood and BALF by ELISA, and content of malonaldehyde (MDA) in lobus inferior pulmonis homogenate by thio barbituric acid colorimetric method were determined. Pathological change of the upper lobe of lung was examined and lung injury was scored as well.</p><p><b>RESULTS</b>As compared with that in the model group, the level of PaO2 was higher in Tet prevention group at 0.5 h, 2 h, 4 h, 6 h and in the Tet treatment group at 2 h, 4 h, 6 h (P< 0.05); and the content of TNF-alpha, MDA and neutrophil percentage in BALF, as well as W/D and lung injury score were lower in the Tet prevention group and the Tet treatment group (P <0.05). Pathological changes of lung in the two Tet groups were all better than those in the model group.</p><p><b>CONCLUSION</b>Early intervention with tetrandrine shows a protectiv effect on rats against acute lung injury induced by intravenous injection of LPS.</p>

Inhibitory effect of emodin on proliferation of human lung adenocarcinoma cells in vitro / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the anti-tumor mechanisms of emodin on human lung adenocarcinoma cell line Anip973 (LACC).</p><p><b>METHODS</b>The influence of emodin of different concentration at different time on LACC proliferation were determined and compared using MTT colorimeric assay and cell growth curve assay. And the cell apoptotic rate was determined by flow cytometry and analyzed with electronic microscope.</p><p><b>RESULTS</b>In a certain range, the higher concentration and longer acting time of emodin could induce the stronger inhibitory effect on tumor cell growth and the higher apoptotic rate. The proliferation inhibitory rate reached 90% after LACC being treated with emodin 60 micromol/L for 72h.</p><p><b>CONCLUSION</b>Emodin can significantly inhibit proliferation of human LACC, showing dose-effect and time-effect relationship.</p>