Résumé
Leptospirosis as a common zoonotic diseases has a worldwide distribution. To Investigate of seroprevalence leptospiral infection in slaughtered camels [Camelus dromedarius] province of Yazd, Iran. Blood samples were collected from 128 camels. Sera were initially screened at serum dilution of 1:100 against six live antigens of [Leptospira interrogans serovars pomona, canicola, hardjo, ballum, icterohaemorrhagiae, grippotyphosa] using microscopic agglutination test. The values >/= 50% in a dilution 1:100 were considered as positive ones. Sera with positive values were titrated against reacting antigens in serial dilutions from 1:100 to 1:400. Antibodies against one or more serovars were shown in 30 [32.4%] sera at dilution >/= 1:100. Among the positive sera, pomona [57.9%] canicola [23.7%], hardjo [10.5%], grippotyphosa [5.3%] and icterohaemorrhagiae [2.6%] were the most frequent serovars,respectively. Furthermore antibodies against more than one serovar were found in 8 [26.6%] of positive sera. It seems that leptospiral with various serovars could be considered as a prevalent infection in camels of the central part of Iran
Résumé
Leptospirosis is a world-wide zoonotic infection occurring in animals and human. Rodents and wildlife are the major sources of the infection which can excrete Leptospira in their urine. The purpose of this study was to assess the seroprevalence of leptospira species in the slaughter house workers in khoy City. 30 serum samples were obtained from people slaughterhouse workers. Sera were tested by MAT using live Leptospira antigens including hardjo, Pomona, icterohaemorrhagiae, grippotyphosa, canicola and ballum. The results of this study showed that four samples [13/33 percent] were positive and 12 samples [40 percent] had doubtful results. Icterohaemorrhagiae and Pomona were the most-prevalent serotypes in positive samples and samples with doubtful results respectively. All positive samples were identified in 1:200 titre. All of the samples were negative in 1:100 and 1:400 dilutions. 50% of the positive sera belonged to the subjects between 20 and 30 years of age and other 50% belonged to those with an age range of 40-50 years. 50% of the positive samples and 83% of the samples with doubtful results belonged to the subjects with employment records of less than 7 years. Leptospirosis is an occupational disease and slaughterhouse workers are more at risk of contamination with different strains of Leptospira and developing this infection which leads to transmission of contamination to protein and nutrient cycling
Résumé
Leptospirosis is one of the zoonotic diseases caused by different serovars of the species Leptospira interrogans. The source of infection in humans is usually either direct or indirect contact with the urine of infected animals. In this study, 400 human blood samples collected from tribal areas of Farsan and Koohrang cities in West Centeral Iran. The serum samples were analyzed employing Microscopic Agglutination Test [MAT]. From 400 Samples, 194 Samples [48.5%] were positive to different leptospiral sreovars at a minimum titre of 1/100. The highest seroprevalence was to serovar hardjo [54.1% of seropositives] and the least was to serovar grippotyphosa [1%]. Seroprevalency of women were higher than men