Fine needle aspiration for flow cytometry immunophenotyping of non Hodgkin lymphoma

The aim was to evaluate the usefulness of lymph node biopsies obtained by fine needle aspiration (FNA) for immunophenotyping of non Hodgkin lymphoma (NHL). Seventeen superficial and deep lymph node samples were fractioned for conventional cytological examination and immunophenotyping studies. Out of ten NHL, nine were readily detected by flow cytometry (FC), while failure on the remaining case was due to selective loss of large cell population, which is liable to occur with this procedure. A single case, which proved negative for all markers employed, was finally diagnosed by immunohistochemistry as germ cell tumor. The other six cases, presenting lymphoid population without phenotypic abnormalities, were diagnosed by cytology and/or histology as Hodgkin disease or hyperpiasic disorders. To conclude, FC immunophenotyping seems to improve the efficacy of FNA in NHL diagnosis, whereas for Hodgkin disease and hyperplasic disorders, classic morphological criteria are more useful for differential diagnosis. Although FNA for FC immunophenotyping cannot replace histopathological examination for NHL diagnosis, it proves to be a useful tool for staging and follow up, making surgical procedures for sample collection unnecesary.

Punción aspiración con aguja fina de mas abdominales bajo control ecografico: diagnostico citologico y experiencia con marcadores biologicos / Aspiration puncture with fine needle of abdominal masses under echographic control: cytological diagnosis and experience with biological markers

Se efectuaran 43 punciones aspiraciones con aguja fina de masas abdominais (hígado, páncreas, colon, vesícula) bajo control ecográfico. El diagnóstico de las muestras se realizó con métodos citológicos de rutina y se efectuó inmunodetección de marcadores biológicos de tumor, de estirpe y origen. Se evaluó la sensibilidad y exactitud del método en el diagnóstico de masas abdominales y se discuten las ventajas de este procedimiento