Résumé
Rickettsia tsutsugamushi organisms were identified in Giemsa and fluorescent antibody stained monocyte cell cultures derived from experimentally infected monkeys and dogs. The identification of organisms in monocyte cell cultures compared favorably with the standard technique of mouse inoculation.
Sujets)
Animaux , Colorants azurés , Chiens , Technique d'immunofluorescence , Haplorhini , Monocytes/microbiologie , Orientia tsutsugamushi/isolement et purification , Fièvre fluviale du Japon/microbiologieRésumé
Over 1,200 isolations of R. tsutsugamushi were obtained over 7 generations of L. (L.) arenicola and no reproducible differences in virulence or antigenic markers could be detected among these isolations. The F8 generation yielded rickettsia with the same antigenic makeup and virulence properties as the original female parent mite. When these isolates were inoculated into mice they elicited the same predominant antibody specificities, i.e. they all produced antibody which reacted with the TA 763 and Karp strains. These were the same as the major antigens detected by direct fluorescent antibody staining of the isolates.
Sujets)
Animaux , Spécificité des anticorps , Antigènes bactériens , Vecteurs arachnides , Mites (acariens)/microbiologie , Orientia tsutsugamushi/immunologie , Trombiculidae/microbiologie , VirulenceRésumé
A high prevalence of murine typhus was found in the human and rodent populations of Senaling, West Malaysia. In addition to 2 index cases which prompted the investigation, 45% of the humans and 35% of the rodents had IFA titers of 1/50 or greater. Serological studies provided evidence of recent infections in 3 human subjects. In a control group of Malaysian soldiers IFA titers of 1/50 or greater were found in only 13 (5%) of 265 sera tested.