Comments on substrate exhaustion during Klebsiella pneumoniae growth

The behavior of the bacterium Klebsiella pneumoniae in the condition of substract exhaustion, in different adaption levels, was studied. The objective was to stablish a substrate exhaustion indicator, for the study of 2,3-butanediol production from eucalyptus hemicellulosic hydrolizate, in fed-batch process. The bacterium was acclimatized in synthetic medium containing 0, 10 and 20 (per cent) of nontreated hydrolizate. The results showed that pH and dissolved oxygen are afected when different levels of adaption are used and that the best substrate exhaustion indicator is the increase of the percentage of dissolved oxygen in the medium.