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1.
Laboratory Animal Research ; : 166-170, 2016.
Article Dans Anglais | WPRIM | ID: wpr-94496

Résumé

Pet turtles are known as a source of Salmonella infection to humans when handled in captivity. Thirty four turtles purchased from pet shops and online markets in Korea were examined to determine whether the turtles and their environment were contaminated with Salmonella spp. Salmonella spp. were isolated from fecal samples of 17 turtles. These isolates were identified as S. enterica through 16S rRNA gene sequencing. The isolation rate of Salmonella spp. from the soil and water samples increased over time. We concluded that a high percentage of turtles being sold in pet shops were infected with Salmonella spp., and their environments tend to become contaminated over time unless they are maintained properly. These results indicate that pet turtles could be a potential risk of salmonellosis in Korea.


Sujets)
Humains , Gènes d'ARN ribosomique , Corée , Prévalence , Salmonelloses , Salmonella , Sol , Tortues , Eau
2.
Korean Journal of Veterinary Research ; : 181-184, 2015.
Article Dans Anglais | WPRIM | ID: wpr-223843

Résumé

Ciliary rootlet coiled coil protein (CROCC), the structural component that originates from the basal body at the proximal end of the ciliary rootlet, plays a crucial role in maintaining the cellular integrity of ciliated cells. In the current study, we cloned Xenopus CROCC and performed the expression analysis. The amino acid sequence of Xenopus laevis was related to those of Drosophila, cow, goat, horse, chicken, mouse and human. Reverse transcription polymerase chain reaction analysis revealed that CROCC mRNA encoding a coiled coil protein was present maternally, as well as throughout early development. In situ hybridization indicated that CROCC mRNA occurred in the animal pole of embryo during gastrulation and subsequently in the presumptive neuroectoderm at the end of gastrulation. At tailbud stages, CROCC mRNA expression was localized in the anterior roof plate of the developing brain, pharyngeal epithelium connected to gills, esophagus, olfactory placode, intestine and nephrostomes of the pronephric kidney. Our study suggests that CROCC may be responsible for control of the development of various ciliated organs.


Sujets)
Animaux , Humains , Souris , Séquence d'acides aminés , Corpuscules basaux , Encéphale , Poulets , Clones cellulaires , Drosophila , Structures de l'embryon , Épithélium , Oesophage , Gastrulation , Branchies , Capra , Equus caballus , Hybridation in situ , Intestins , Rein , Plaque neurale , Réaction de polymérisation en chaîne , Transcription inverse , ARN messager , Xenopus laevis , Xenopus
4.
Journal of Veterinary Science ; : 139-145, 2004.
Article Dans Anglais | WPRIM | ID: wpr-128640

Résumé

Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the N. caninum proteome. Protein sample preparation was carried out by first conducting sonication, followed by adding lysis buffer containing 7M urea plus 2M thiourea to the purified tachyzoites in order to complete disruption. A total of 335 differentially expressed protein spots were detected using pH 4-7 IPG strip (7 cm) that were run in a 56 kVh isoelectric focusing (IEF) system. Of the spots analyzed, 64 were identified as antigenic spots on immunoblot profile. Major antigenic spots appeared at 65 kDa (pI 5.2-5.3), 51 kDa (pI 5.5), 38 kDa (pI 5.1), 33 kDa (pI 4.4), 29 kDa (pI 5.6) and 15.5 kDa (pI 5.0) were observed to be significantly distinct compared to the rest of the antigenic spots. The results indicate that combination of 2-DE and immunoblotting methods were thought as very useful tools in defining both proteins and antigens of N. caninum tachyzoites. Additionally, present 2-DE profiles may be valuable in further proteomic approaches and study of the pathogen.


Sujets)
Animaux , Production d'anticorps , Antigènes de protozoaire/analyse , Électrophorèse bidimensionnelle sur gel/méthodes , Électrophorèse sur gel de polyacrylamide , Épitopes/analyse , Traitement d'image par ordinateur , Immunotransfert/méthodes , Focalisation isoélectrique , Neospora/composition chimique , Protéome/analyse , Protéomique , Protéines de protozoaire/analyse
5.
Journal of Veterinary Science ; : 387-390, 2004.
Article Dans Anglais | WPRIM | ID: wpr-79774

Résumé

Black rockfish (Sebastes schlegeli) is an important mariculture species in Korea. The production of this fish is drastically declined due to bacterial diseases, particularly streptococcosis caused by Lactococcus garvieae. The bacterial surface characteristics of SJ7 and TY6 were found to have capsule but not NB13 and YS18. The experiential evaluation of L. garvieae pathogenicity, the capsular isolates showed high cumulative mortality i.e. SJ7 (100%) and TY6 (60%) compared to non-capsular isolates. Based on this result the capsular isolates L. garvieae were highly suspected as the causative agent of streptococcosis in rockfish.


Sujets)
Animaux , Tests d'agglutination/médecine vétérinaire , Capsules bactériennes , ADN bactérien/génétique , Maladies des poissons/microbiologie , Poissons , Technique d'immunofluorescence indirecte/médecine vétérinaire , Infections bactériennes à Gram positif/microbiologie , Lactococcus/pathogénicité , Réaction de polymérisation en chaîne/médecine vétérinaire
6.
Journal of Veterinary Science ; : 143-149, 2003.
Article Dans Anglais | WPRIM | ID: wpr-105183

Résumé

Protein identification by peptide mass fingerprinting using matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analyze unambiguously identity of the spots from a 2-dimensional electrophoresis (2-DE) gel. This study developed a technique for 2-DE of Salmonella enterica serovar Enteritidis (S. enteritidis) by improving the dissolution conditions by 2-DE using a pH 4 - 7 immobilized pH gradient (IPG) strip. This report examines the protein components from the patterns of the S. enteritidis protein. The most abundant protein displayed a great number of clusters within the pH 4.5 - 7 range with a molecular mass ranging from 35-80 kDa. Some of these spots were identified as metabolic related enzymes. The protein fraction was also analyzed using an immobilized pH gradient strip. Different proteins were identified on the spot according to the elongation factors. In addition, this study showed that the 2-DE analysis of S. enteritidis provides useful information regarding the S. enteritidis proteome, and this approach might provide a strategy for identifying bacterial proteins using a proteome technology.


Sujets)
Protéines bactériennes/analyse , Électrophorèse bidimensionnelle sur gel , Enzymes/composition chimique , Masse moléculaire , Salmonella enteritidis/composition chimique , Spectrométrie de masse MALDI
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