Determination of cultural conditions for the production of rhamnolipid biosurfactant by pseudomonas aeruginosa RB 28

The ability of [20] bacterial isolates of the genus Pseudomonas isolates was tested to produce biosurfactant using crude oil as carbon source. P.aeruginosa RB28 was selected for its high ability to produce biosurfactant. The optimal conditions for production of biosurfactant from RB 28 strain were studied, the results showed that sunflower oil [4%], urea [6g/l], Mg[++] [0.5g/I], Fe[++] [0.008 g/1], Ca[++] [0.05g/l], pH 7.0 and temperature between 30-34 °C after 60 hours of incubation. The maximum production of rharnnolipid [7g/l] was obtained after using these optimum conditions