Detection of high molecular weight IgG fibronectin complexes in various types of Acute Myeloid Leukemia – A study in third world country.

To detect presence of high molecular weight complexs of lgG and fibronectin, plasma of patients with acute myloid leukemia was examined by polyethylene glycol (PEG) precipitation, analytical ultracentrifugation, and and immunoaffinity chromatography. ultracentrifugation identified abnormal circulating high molecular Weight lgG in all patients. this was precipitated by PEG and was shown by exclusion chromatography to contain IgG in a high molecular weight form. Examination of plasma by immunoaffinity chromatography supported evidence for complex formation between IgG and fibronectin and further showed that abnormal high molecular weight IgG complexes are a prominent feature of Acute Myeloid Leukemia and implicate lgG fibronectin complex formation.

Development, characterization and solubility study of solid dispersion of nifedipine hydrochloride by solvent evaporation method using poloxamer 407.

The present research endeavor was towards the enhancement of solubility of nifedipine by solid dispersion method, were prepared by solvent evaporation method and polymers Poloxamer 407 was tried with different proportion with drug and increasing the different sodium lauryl sulphate (SLS) percentages. There was significant increase of dissolution rate of nifedipine, in SD of nifedipine + Poloxamer 407 (1:4) than nifedipine + Poloxamer 407 (1:2) and nifedipine + Poloxamer 407 (1:3). Solid dispersion of nifedipine was evaluated by solubility test, DSC, IR and dissolution characteristics. Solid Dispersion of Nifedipine in Poloxamer 407 improved the dissolution rate of nifedipine, which helps to enhancing solubility of Nifedipine. Dissolution rate of pure nifedipine increased, with increasing the various Polymers content and with increasing the various sodium lauryl sulphates (SLS) content. The solubility of pure nifedipine was observed in pH 7.2 ± 0.2 buffer solution, with increasing the polymer ratio as 1:2, 1:3, 1:4 and with increasing the SLS percentage(%) as 1%, 3% and 5% respectively.