RÉSUMÉ
[Objective]To investigate the meaning of dietary nursing theory of Huangdi Neijing,and provide a daily diet guideline for patients with systemic lupus erythematosus(SLE).[Methods]Based on the diet theory of Huangdi Neijing,consulting ancient books and modern research,analyze the relationship between diet and SLE,sum up the influence of diet on disease.[Results]SLE patients should restrict calories appropriately,shorten feeding time,adjust diet structure.Besides,consumption of spicy food such as chili and garlic based on disease syndrome or physique,would improve body metabolism and inflammation.At the same time,patients should reduce the consumption of foods rich of sugar and fat,which always cause skin injury,disorder of glucose and lipid metabolism,also induce imbalance of intestinal flora.Moreover,high sugar is linked to joint pain and hair loss,and high fat also aggravates intestinal inflammation.Finally,it is advised that patients with joint sore having food rich in vitamin D or type Ⅱ collagen,consumption of tea and coffee also have an antioxidant effect,and consumption of yogurt and cheese is conducive to regulate the intestinal flora.[Conclusion]Diet nursing is one of the most characteristic theories in Huangdi Neijing,which can effectively guide the daily diet of SLE patients,help them to alleviate the disease,reduce the side effects of drugs,and play a certain auxiliary role in the treatment of diseases,Huangdi Neijing diet theory can be used as the guiding principle of daily diet for SLE patients.
RÉSUMÉ
<p><b>OBJECTIVE</b>To explore efficacy enhancing and detoxification roles of Jiedu Quyu Zishen Recipe (JQZR) in treating systemic lupus erythematosus (SLE) by studying its effect on Toll like receptor 9 (TLR9) signal pathway of murine macrophage cells after JQZR stimulated CpG oligodeoxynucletide (CpG ODN).</p><p><b>METHODS</b>Murine macrophage cells in vitro cultured were randomly divided into 4 groups, i.e., the blank serum group, the CpG ODN stimulus group, the CpG ODN + dexamethasone group, the CpG ODN + medicated serum group. Murine macrophage cells were collected after 24-h intervention. The expression of TLR9, myeloid differentiation factor 88 (MyD88), NF-KB, IFN-α mRNA were analyzed by RT-PCR. The expression of TLR9 and NF-κB protein were analyzed by Western blot. Changes of the NF-KB transcriptional activity were assayed by Dual-Luciferase reporter assay system.</p><p><b>RESULTS</b>mRNA expressions of TLR9, MyD88, NF-κB, and IFN-α, protein expressions of TLR9 and NF-κB, and NF-κB transcriptional activities were enhanced, showing statistical difference when compared with those of the blank serum group (P <0. 05, P <0. 01). Compared with the CpG ODN stimulus group, mRNA expressions of MyD88, NF-κB, and IFN-α, the protein expression of NF-κB and the NF-κB transcriptional activities decreased in the CpG ODN + dexamethasone group with statistical difference (P <0. 01). Compared with the CpG ODN stimulus group, mRNA expressions of TLR9, MyD88, NF-κB, and IFN-α, protein expressions of TLR9 and NF-κB, and NF-κB transcriptional activities were decreased in CpG ODN+ medicated serum group with statistical difference (P <0. 01).</p><p><b>CONCLUSION</b>Efficacy enhancing and detoxification roles of JQZR in treatment of SLE might be realized through regulating TLR9 signal pathways.</p>