Résumé
Objective: To observe the effect of the administration temperature of GQD on the efficacy to ulcerative colitis (UC) of rats. Methods: The GQD was given to dextran sulfate sodium (DSS)-induced UC rats at different temperature (10℃/42℃) for 7 d. The disease activity index (DAI), histopathological score of colon, superoxide dismutase (SOD), malondialdehyde (MDA), GHS-Px, myeloperoxidase (MPO) of serum and colon tissue, and the tumor necrosis factor (TNF-α) of serum were measured and observed. Results: Compared with the control group, the UC relative symptom and index changes of rats in the model group appeared obviously. GQD showed the significant therapeutic effects in both 10℃ and 42℃ groups. The lower temperature administration achieved significantly better results than the higher one on the histopathological score, colon wall thickness, and biochemical indices. Conclusion: The cold GQD shows higher efficacy than the hot GQD when treating UC, which may be attributed to the immune response of intestinal mucosal mast cells to temperature-induced stress.
Résumé
The combination of Glycyrrhizae Radix et Rhizoma and Aconiti Lateralis Radix Preparata can increase efficacy and decrease toxicity. This study started from the phenomena of protein self-assembly in the mixed decoction of Glycyrrhizae Radix et Rhizoma with Aconiti Lateralis Radix Preparata. The attenuated mechanism was explored between the combination of Glycyrrhizae Radix et Rhizoma and Aconiti Lateralis Radix Preparata by using the protein of Glycyrrhizae Radix et Rhizoma and aconitine which was the major toxic component of Aconiti Lateralis Radix Preparata. Glycyrrhizae Radix et Rhizoma protein with aconitine could form stable particles which particle mean diameter was (206.2 ± 2.02) nm and (238.20 ± 1.23) nm at pH 5.0 in normal temperature. Through the mouse acute toxicity experiment found that injection of aconitine monomer all mice were killed, and injection of Glycyrrhizae Radix et Rhizoma protein-aconitine particles with the same content of aconitine all mice survived. Survey the stability of Glycyrrhizae Radix et Rhizoma protein-aconitine shows that the colloid particles is stable at room temperature, and it has the possibility to candidate drug carrier. Glycyrrhizae Radix et Rhizoma protein can reduce the toxicity of aconitine through self-assembly.