Study on the correlation between the time within the glucose target range, the level of glycosylated hemoglobin and the risk of diabetic retinopathy / 中华眼底病杂志

Objective:To observe and analyze the correlation between time within target glucose range (TIR) and hemoglobin A1c (HbA1c) and the risk of diabetic retinopathy (DR).Methods:A retrospective clinical study. From March 2020 to August 2021, 91 patients with type 2 diabetes mellitus (T2DM) who were hospitalized in Department of Endocrinology and Metabolic Diseases, Affiliated Hospital of Weifang Medical University, were included in the study. All patients underwent Oburg's no-dilatation ultra-wide-angle laser scan ophthalmoscopy, HbA1c and continuous glucose monitoring (CGM) examinations. According to the examination results and combined with the clinical diagnostic criteria of DR, the patients were divided into non-DR (NDR) group and DR group, with 50 and 41 cases respectively. The retrospective CGM system was used to monitor the subcutaneous interstitial fluid glucose for 7 to 14 consecutive days, and the TIR was calculated. Binary logistic regression was used to analyze the correlation between TIR, HbAlc and DR in patients with T2DM0. At the same time, a new indicator was generated, the predicted probability value (PRE_1), which was generated to represent the combined indicator of TIR and HbA1c in predicting the occurrence of DR. The receiver operating characteristic curve (ROC curve) was used to analyze the value of TIR, HbAlc and PRE_1 in predicting the occurrence of DR.Results:The TIR of patients in the NDR group and DR group were (81.58±15.51)% and (67.27±22.09)%, respectively, and HbA1c were (8.03±2.16)% and (9.01±2.01)%, respectively. The differences in TIR and HbA1c between the two groups of patients were statistically significant ( t=3.501,-2.208; P=0.001, 0.030). The results of binary logistic regression analysis showed that TIR, HbA1c and DR were significantly correlated (odds ratio=0.960, 1.254; P=0.002, 0.036). ROC curve analysis results showed that the area under the ROC curve (AUC) of TIR, HbA1c and PRE_1 predicting the risk of DR were 0.704, 0.668, and 0.707, respectively [95% confidence interval ( CI) 0.597-0.812, P=0.001; 95% CI 0.558-0.778, P=0.006; 95% CI 0.602-0.798, P=0.001]. There was no statistically significant difference between TIR, HbA1c and PRE_1 predicting the AUC of DR risk ( P>0.05). The linear equation between HbAlc and TIR was HbAlc (%) = 11.37-0.04×TIR (%). Conclusions:TIR and HbA1c are both related to DR and can predict the risk of DR. The combined use of the two does not improve the predictive value of DR. There is a linear correlation between TIR and HbAlc.

Comparison of the intraradicular bacterial community structures of teeth with or without post-treatment periapical periodontitis / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To compare the intraradicular bacterial community structures of teeth with or without post-treatment periapical periodontitis and to explore the suspicious microorganisms that is related to persistent periapical infection.</p><p><b>METHODS</b>The intraradicular biofilm samples were collected from 10 post-treatment periapical periodontitis teeth (apical periodontitis group) and 10 teeth without post-treatment periapical periodontitis (without apical periodontitis group). The V1-V3 hypervariable regions of bacterial 16S rRNA genes were amplified, and the high-throughput pyrosequencing was performed. The composition and structure characteristic of intraradicular microbiome were revealed by bioinformatic analysis.</p><p><b>RESULTS</b>Total sequences were taxonomically classified into 132 species-level bacteria belonging to 96 genera and 21 phyla. The most representive phyla in apical periodontitis group were Firmicutes [32% (18 534/58 688)], Proteobacteria [27% (15 626/58 688)], Actinobacteria [15% (8 685/58 688)], Bacteroidetes [11% (6163/58 688)], Fusobacteria [8% (4761/58688)] and Spirochaetes [3% (1 785/58 688)]. While the most representive phyla in without apical periodontitis group were Firmicutes [31% (16 941/55 480)], Proteobacteria [27% (14 748/55 480)], Bacteroidetes [18% (9 948/55 480)], Fusobacteria [10% (5 307/55 480)], Actinobacteria [9% (4 761/55 480)], Chloroflexi [3% (1 785/55 480)]. The abundance of actinobacteria in apical periodontitis group was significantly higher than without apical periodontitis group (P < 0.01). The detection rates of actinomycetes in apical periodontitis group and without apical periodontitis group were 100% and 50%.</p><p><b>CONCLUSIONS</b>The diversity of intraradicular bacterial community in teeth with apical periodontitis was higher than those without apical periodontitis. Actinomycetes may be related to post-treatment periapical periodontitis.</p>

Induced differentiation of rat ectomesenchymal cells to odontoblast-like cells——Setup of three-dimension culture model / 实用口腔医学杂志

Objective: To study the mechanism of differentiation of rat ectomesenchymal cells to odontoblasts. Methods: Ectomesenchymal cells were cultured in three-dimension culture model using collagen gel as frame, and the change of phenotype of ectomesenchymal cells were observed and detected by phase-contrast microscopy and immunohistochemistry after the cells had been treated by 10 ng/ml of bFGF or/and 100 ng/ml IGF-1. Results: 4 days after treatment by bFGF and IGF-1, the cells appeared to be odontoblast-like cells aligned parallelly and polarized with long cytoplasmic processes attached to one end of the cell body.The cells were positive for DSP expression. However, the cells were DSP negative and aligned disorderly in other groups. Conclusion: Ectomesenchymal cells can be induced to differentiate to odontoblast-like cells in three-dimension culture model with the treatment by bFGF and IGF-1.

Interleukin-1? concentration in apical exudates of periapical periodontitis and its correlations with the clinical findings / 实用口腔医学杂志

Objective: To compare the concentration of IL-1? in the apical exudates of phoenix abscess of chronic periapical periodontitis and to examine the correlation of IL-I? concentration with clinical and radiographic findings of the involved teeth. Methods: 35 single-rooted teeth diagnosed as phoenix abscess and 35 as chronic periapical periodontitis were examined. The periapical signs and symptoms were recorded. Radiographs were taken and periapical radiolucent areas were calculated with the help of the AutoCAD software. The standard paper-point sampling method was used to collect and quantify the periapical exudates. IL-1? in the exudates was detected by enzyme-linked immunosorbent assay (ELISA). All statistical analyses were finished with SPSS 10.0 software. Results: The phoenix abscess group showed significantly lower concentration(5.65?2.76) ng/ml of IL-1? in the exudates and larger radiolucent areas(32.10?13.82) mm 2 on the X-ray films than the chronic periapical periodontitis group[(12.51?5.15) ng/ml and (6.51?3.56) mm 2 respectively] (P