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Objective To explore the expression of heat shock protein (HSP) 105b in advanced adenocarcinoma of esophagogastric junction (AEG) patients and its relation with clinicopathological characteristics and effect of hyperthermic intraperitoneal chemotherapy (HIPEC). Methods We randomly divided 166 cases of advanced AEG who underwent open radical gastrectomy and lymphadenectomy D2 compartment into treatment group (prophylactic HIPEC of paclitaxel after operation) and control group (conventional treatment). Immunohistochemistry was used to detect HSP105b expression in postoperative tumor tissues, and to analyze its relation with clinicopathological characteristics and effect of HIPEC. Results The expression of HSP105b was only associated with tumor vein infiltration (t=4.002, P=0.045). The 3-year disease-free survival rate of the patients with high HSP105b expression was significantly lower than those with low HSP105b expression (56.5% vs. 64.8%, χ2=35.508, P < 0.001), and the disease-free survival rate of the patients with high HSP105b expression in treatment group was significantly lower than that with low HSP105b expression (60.7% vs. 71.5%, χ2=77.459, P < 0.001). Conclusion HSP105b can be used as a prognostic factor and its expression can predict the efficacy of HIPEC in the patients with advanced AEG.
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OBJECTIVE： To investigate the effects of Buyang huanwu decoction on the expression of MMPs and TIMPs in cardiac tissue of viral myocarditis （VMC） model mice. METHODS： Male BALb/c mice were randomly divided into control group， model group， positive control group [captopril， 100 mg/（kg·d）]， Buyang huanwu decoction low-dose， medium-dose and high-dose groups [6， 18， 36 g/（kg·d）]， with 24 mice in each group. Except for control group， other groups were given Coxsackie virus B3 once intraperitoneally to induce VMC model. After modeling， control group and model group were given constant volume of normal saline intragastrically； administration groups were given relevant medicine intragastrically； once a day， for consecutive 30 days. The general situation of mice in each group was observed. The day of inoculation was set at 0 d， heart mass to body mass ratio （HW/BW） was measured at 4， 10， 20， 30 d after inoculation. The morphological characteristics of myocardium were observed by HE staining， and the myocardial histopathological scores of myocardium were evaluated. The distribution of type Ⅰ and Ⅲ collagen in myocardium was observed by Abcam picrosirius red staining， and the ratio of type Ⅰ to Ⅲ collagen was calculated. At 30 d， relative expressions of MMP-1， MMP-3， MMP-9 and TIMP-1 in cardiac tissue were detected by Western blotting assay， and the ratio of MMPs to TIMPs was calculated. RESULTS： Compared with control group， mice in model group suffered from irritability， arch back， alleviation of stimulation response， reduction of body mass and even mental depression. Typical inflammatory changes and local interstitial hyperemia were observed in the myocardium， accompanied by a large number of lymphocyte infiltration and distribution of type Ⅰ and Ⅲ collagen. HW/BW （at different time points of 10-30 d）， myocardial histopathological score （at different time points of 4-30 d）， ratio of type Ⅰ and Ⅲ collagen （at different time points of 4-30 d）， the expression of MMP-1 and MMP-9， ratio of MMPs to TIMPs were increased significantly， while the expression of MMP-3 and TIMP-1 were decreased significantly （P＜0.05）. Compared with model group， above symptoms of mice in administration groups were improved to different extents. HW/BW [at different time points of 10-30 d in administration groups （except for 10 d in Buyang huanwu decoction low-dose group）]， myocardial histopathological score （at different time points of 10-30 d in administration groups）， ratio of type Ⅰand Ⅲ collagen （at different time points of 4-10 d in positive control group and Buyang huanwu decoction high-dose group， at different time points of 20-30 d in Buyang huanwu decoction low-dose and medium-dose groups）， the expression of MMP-1 （positive control group and Buyang huanwu decoction high-dose group） and MMP-9 （administration groups）， ratio of MMPs to TIMPs （administration groups） were decreased significantly， while the expression of MMP-3 （positive control group， Buyang huanwu decoction low-dose and high-dose groups） and TIMP-1 （administration groups） were increased significantly （P＜0.05）. CONCLUSIONS： Buyang huanwu decoction can inhibit myocardial fibrosis of VMC model mice by inhibiting myocardial collagen hyperplasia， regulating the expression of MMPs and TIMPs， improving MMPs/TIMPs imbalance.
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Objective To investigate the clinical significance and changes of neuron-specific enolase (NSE) and S100 protein in blood serum in patients with enterovirus 71 infection.Methods A total of 176 children with enterovirus 71 infection admitted from March 1,2012 through October 31,2012 were enrolled for a prospective and control study.According to diagnostic criteria of the enterovirus 71 (EV71) infection instituted by expert consensus for treatment of severe patients sets in 2011,the patients were divided into three groups:mild group (n =62),severe group (n =65) and critically care group (n =49),and another 30 healthy children served as control group.The demographics of patients including age and sex were comparable between control group and the sick children groups.Four milliliter of peripheral blood were taken from ill children on the first day before treatment and on the first,second,third day after treatment.The blood samples of healthy children were taken on the first day after physical examination.At the same time,the clinical data of blood routine,blood biochemistry,myocardial enzymes and C-reactive protein during the first 24 hours were collected.Immunohistochemical technique was used to study the change of NSE and S100 levels in serum.Data were expressed in mean ± standard deviation ((x) ± s) and were statistically analyzed by using SPSS 13.0 statistical software.Comparisons were carried out among different groups with one way analysis of variance (ANOVA) and between groups were performed with the Student t test.Changes were considered as statistically significant if P values was less than 0.05.Results ①Compared with mild group and control group,the levels of NSE and S100 protein were significantly higher in severe group and critically care group (P ＜0.05).②The serum levels of NSE and S100 protein in severe group were higher than in those in mild group with better outcomes (P ＜ 0.05).Conclusion The serum levels of NSE and S100 protein as biomarkers can be used to evaluate the severity of EV71 infection,and can also be used to determine the efficacy of treatment.
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Objective To investigate the effect of health education on enhanced curative effect of motor imagination therapy of patients with hyperbaric oxygen(HBO). Methods Thirty patients undergoing HBO combined with motor imagination therapy were given health education including contents of motor imagination,time for motor imagination,intervention of bad emotions and time for motor imagination after leaving the treatment cabin.The understanding of motor imagination-associated knowledge after education was compared to that before education. Results The understanding of motor imagination-associated knowledge after education was significantly better than before education(χ2=9.017,P=0.003). Conclusion Health education may help improve the curative effect of hypterbaric oxygen treatment(HBO)by way of improving the understanding of motor imagination-related knowledge.
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@#ObjectiveTo observe the clinical effect of oral drugs associated with acupuncture on peripheral nervous lesions of diabetes mellitus.Methods60 diabetes mellitus patients with peripheral nervous lesions were randomly divided into the treatment group and control group with 30 cases in each group. Patients of the treatment group were treated with oral drugs and acupuncture, but cases of control group were only taking oral drugs.ResultsThe effective rate of the treatment group was 86.67% higher than that of the control group (43.33%)(P<0.01).ConclusionDrugs associated with acupuncture has a good effect on peripheral nervous lesions of diabetes mellitus.
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@#ObjectiveTo evaluate the therapeutic effect of three different recovery treatments on herniation of lumbar intervertebral disc.Methods156 cases were divided into three groups, traction of lumbar (Group A, n=52), traction compared with physical therapy(Group B, n=52), traction with massage and electroacupuncture therapy(Group C,n=52). Before and after treatment, the clinical symptoms and signs were assessed and compared. ResultsThe efficiency of alleviative pain and the index of function improvement of lumbar vetebra were higher in Group B, C than that in Group A (P<0.05), higher in Group C than that in Group B (P<0.05).ConclusionThe traction of lumbar with massage and electroacupuncture is preferably effective, and the manual reduction plays an important role.
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@#ObjectiveTo study the effect of facilitation techniques combined with acupuncture on movement function and activity of daily living(ADL) of the stroke patients.Methods200 patients with stroke were divided randomly into 4 groups,group A for facilitation techniques with acupuncture,group B for facilitation techniques only,group C for acupuncture therapy only,and group D for control.The recovery grades of the Brunnstrom and Functional Independent Measure(FIM) were applied to assess movement function and ADL before and after treatment.ResultsThe number of patients whose limbs recoveried up to Brunnstrom 4 in group A、B、C after treatment was higher than that in group D as while as the scores of FIM(P<0.05).The FIM scores and the number of patients whose whose limbs recoveried grades(>4 grades) of the Brunnstrom in group B and C were no difference(P>0.05),The FIM scores and the number of patients whose recovery grades(>4 grades) of the Brunnstrom in group A were significant higher than that in group B and C (P<0. 05).ConclusionsFacilitation techniques combined with acupuncture can improve the movement function and ADL of the stroke patients.
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@#ObjectiveTo study the effects of early intervention on physical psychomotor development of premature infants.Methods120 survival premature infants were divided into two groups randomly.The infants in intervention group accepted early intervention according to the Early Intervention Program of 0-2 Years Infants,while that in the control did not. The physical and neurological condition and intelligence were tested periodically till 2 years old.ResultsThere were no significant differences between the two groups on physical development.The mental development index(MDI) and psychomotive development index(PDI) in intervention group showed higher than those in control group (P<0.05 and P<0.01 respectively). The abnormal rate of MDI and PDI,the incidence of cerebral palsy in intervention group was lower obviously than that in control group (P<0.01).ConclusionsThe application of early intervention could improve the prognosis of premature infants.