A clinical study of tension-free inguinal hernioplasty in patients with different ages / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the application of mesh and hernia ring for tension-free hernia repair in inguinal hernioplasty in patients with different ages.</p><p><b>METHODS</b>A total of 190 patients with inguinal hernia undergoing tension-free hernia repair were divided into two groups with age above and below 60 years, and the surgical effect and complications were compared.</p><p><b>RESULTS</b>Tension-free hernia repair with mesh and hernia ring was superior to conventional hernia repair in terms of operative time, mean time for exercise and pain in patients above 60 years, but not in patients below 60 years.</p><p><b>CONCLUSION</b>Surgical repair of inguinal hernia should be performed with individualized surgical plans.</p>

Distribution of neuronal nitric oxide synthase-immunopositive neurons in rat corpus striatum and their ultrastructures / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the distribution of neuronal nitric oxide synthase (nNOS)-immunopositive neurons in rat corpus striatum and their ultrastructural features.</p><p><b>METHODS</b>Brain tissue specimens were obtained from normal SD rats, in which nNOS-immunopositive neurons were visualized by ABC immunocytochemistry and observed under immunoelectron microscope with pre-embedding staining.</p><p><b>RESULTS</b>Under light microscope, nNOS-immunopositive neurons appeared brown with distinct profiles of the cell body and processes. These neurons, mostly medium-sized and small cells, were located mainly in the lateral region of the corpus striatum. Only a few immunopositive neurons were detected in the medial region of the corpus striatum. Immunohistochemistry and transmission electron microscopy identified the nNOS-immunopositive neurons as interneurons possessing large nuclei with small amount of cytoplasma. The immunopositive granules were visualized as black plaques, and the larger ones distributed mainly in the cell bodies, some with monolayer membrane encapsulation. The small granules did not have the encapsulation, scattering in perinuclear regions and under the cell membrane, but not in the cell body. The immunopositive granules were also found in the axons and dendrites, but not in the vesicles of the synapses. In addition, many immunopositive terminals were found close to the blood vessels.</p><p><b>CONCLUSIONS</b>nNOS-immunopositive neurons in rat corpus striatum are mainly medium-sized and small cells as is typical of the interneurons. The immunopositive granules locate in the cytoplasma, axons and dendrites, and larger granules have membrane coating while small ones do not, possibly in relation to their functions.</p>