Résumé
Hepatitis C viral RNA in sera from 10 patients with post-transfusion hepatitis in Shanghai (China) were amplified By the nested PCR with primer sets deduced from the American prototype HCV RNA 5' -untranslated region. Eight of 10 cases (80%) were positive. And manipulations of PCR were simplified by combination of reverse transcription step and the first round PCR reaction. Our results indirectly proved that 5-untranslated region of HCV RNA of Shanghai showed a high degree of comservation and homology compared with isolates from some regions in the world.
Résumé
In this study, authors observed and compared the clearances of different molecule weight substances between different dialysis membranes and different hemopurification methods. The results showed that the clearances of BUN by hemodialysis (HD) with cuprophan membrane, hemodiafiltration (HDF) with polysulfone and high flux dialysis (HFD) with polysulfone were all satisfactory. TACurea were all below 50 mg. Hemodialysis with cuprophan could clean out some middle molecule substances (MMS), but not ?2-microglobulin (?2m). Hemodiafiltration with polysulfone had the best clearances of MMS and ?2m. Hemofiltration (HF) with polysulfone had fair clearances of MMS and ?2m, but the clearances of BUN and Cr by HF were not so good. The clearances of MMS and ?2m by high flux dialysis were lower than those of HDF and HF. The clearances of MMS and ?2m by continuous ambulatory peritoneal dialysis (CAPD) at a single time were lower than those of HF, HDF and HFD, but the general clearances of MMS and ?2m by CAPD per week were two to four times higher than those by hemodialysis with cuprophan. CAPD is a choice method for those hospitals where HF or HDF can not be performed.