Résumé
Objective: To observe the level of pro-opiomelanocortin (POMC) mRNA expression in the hypothalamic arcuate nucleus in young and senile rats. Methods: POMC mRNA was determined with in situ hybridization, the hybridization signals and areas of POMC mRNA-positive cells were measured with computer image analysis system. Results: Compared with young rats, average gray values of POMC mRNA-positive neurons in the hypothalalmic arcuate nucleus and near region in the senile rats decreased, but the numbers of POMC mRNA-positive neurons did not change significantly. Conclusion: The decrease of POMC mRNA expression level in individual neurons in hypothalamic arcuate nucleus of senile rats is one of the causes that decrease the expression of peptides-derived from POMC gene.
Résumé
A new menthod for treating old laceration of Lacrimal canaliculus with dacryocystoplasty using conjunctiva or labial mucosa was recommended by the authors. Out of 14 cases,9 cases were operated on with conjunctiva.7 cases were markedly effec- tive,1 cases improved and 1 cases failed.5 cases were operated on with labial mucosa.4 cases were markdly effective and 1 cases improved. The advantages of these two kinds of transplants have been compared.Labial mucosa is better than conjunctiva,and dacryocystoplasty with labial mucosa is more handy and appli- cable.
Résumé
0.05), but the number of VP neurons increased in some neural nuclei while declined in the others. The number of VP neurons in supraoptic nucleus (SO) increased significantly (P
Résumé
The distribution and numbers of SRIF-immunoreactive cell bodies and fibers in normal and castrated rats hypothalamus were studied by PAP immunocytochemistry. The results showed that the positive cell bodies and fibers in periventricular nucleus (PV), suprachiasmatic nucleus (SCN) and paraventricular nucleus (PVN) were more numerous in male than in female. Another marked sexual dimorphism was found in medial preoptic area (MPO) which was filled with dense plexus of varicose immunoreactive fibers in male, while there were low density of stained fibers in female. Orchiectomized rats had fewer number of positive neurons in PV, PVN, SCN and arcuate nucleus (ARN), and lower density of fiber in SCN, ARN, MPO and median eminence than the normal rats. On the contrary, in the ovariectomized rats could find more numerous cell bodies and fibers in these areas. When gonadal steroid was given after gonadectomy, the SRIF neurons became similar to the normal.
Résumé
Cell bodies of the hypothalamus which project to various subdivisions of theparamedian midbrain were studied in the rat with the method of retrograde axonaltransport of HRP.After small amount of HRP was delivered electrophoreticallyinto the dorsolateral part of the central grey substance,the HRP-labelled cells werefound in the anterior hypothalamic nucleus,the ventromedial nucleus,the dorsome-dial nucleus,the dorsal premammillary nucleus,the ventral premammillary nucleus,the medial mammilary nucleus and the lateral mammillary nucleus,mainly in theipsilateral side.Following introduction of the enzyme into the ventral part ofcentral grey substance,there were HRP-labelled cells in the ipsilateral preoptic area,the lateral area of anterior hypothalamus(dorsal to the supraoptic nucleus),thelateral area of tuberal part,the ventromedial nucleus,the dorsomedial nucleus andthe dorsal premammillary nucleus.By introducing the HRP into the medial part oftegmentum,the HRP-labelled cells were observed in the preoptic area,the lateralarea of anterior hypothalamus,the ventromedial nucleus,the dorsomedial nucleus,the dorsal premammillary nucleus,the medial mammillary nucleus and the lateralmammillary nucleus.