Preparation and evaluation of newcastle disease virus inactivated vaccines adjuvanated with crude or fractionized Nigella sative oil

Different types of inactivated oil emulsion Newcastle disease vaccines were prepared using different extractions of the Nigella Sativa oil. The physical properties of emulsions were earned out and included emulsion type, emulsion stability and emulsion viscosity. The vaccinated chicks were bled at one-week intervals post-vaccination over six weeks and the collected sera were tested by the HI test. After that, they were challenged 21-days and 42-days post-vaccination by the intramascular inoculation with VVNDV. From this study we can conclude that the non-specific immunostimulant effect of Nigella Saliva oil is acquired when it is used as a crude oil and this improved its ability as a good adjuvant for viral vaccines