Résumé
In our country, the Wright test routinely is used for diagnosing brucellosis. Because of its low sensitivity, the range of false-negative results is high. Therefore, we aimed at comparing Wright and ELISA in the people suspected brucellosis. The results of Wright, 2MB, Coombs Wright tests were compared with Anti-Brucella IgG, Anti-Brucella IgM. Of 1183 subjects referred for Wright test, 148 of them were investigated for Coombs Wright and 228 for 2MB Wright. In addition to Wright test for 32 cases, Brucella IgG and IgM classes were also experimented. Wright test was negative in 95.4% of cases. Of these negative results, 2.3% were positive for Coombs Wright. Eight-point-five percent of the cases were positive for Coombs Wright test and 4.7% for 2MB Wright test. Sixteen cases were negative for both Wright and ELISA. In 8 cases of Wright-negative, ELISA IgM class was positive and IgG class was negative, and in 4 cases of Wright-negative, ELISA IgM was negative and IgG was positive. About 4 cases of Wright-positive, IgM and IgG antibody classes were positive. Due to the mismatch between the results of Wright agglutination test and ELISA method and with regard to availability, high sensitivity and determining the type of antibody classes in ELISA, it is focused on ELISA method for brucellosis diagnosis
Résumé
Some studies on human beings have suggested that during normal pregnancies an increase in the number of Th2 cells and in women with recurrent spontaneous abortions [RSA] an increase in Th1 cells takes place. The Cross-link of CD26 and CD3 on T-cells with immobilized monoclonal antibodies results in T-cell proliferation and IL-2 [Th1 cytokine] production. CD30 has been described as being preferentially expressed, and sCD30 preferentially released by human T-cells that produce Th2-type cytokines. The objective of this study was to determine whether serum levels of soluble CD26 [sCD26] and CD30 [sCD30] as markers of Th1 and Th2, alter in patients with a history of recurrent spontaneous abortion [RSA], and whether there is any correlation between cytokine production by stimulated peripheral blood mononuclear cells [PBMCs] and serum levels of soluble CD26 and CD30. This was a case-control study on two different groups of people referred to Yazd Research and Clinical Center for Infertility. The case group consisted of 21 women with at least 3 abortions. The participants were visited on the day of their last abortion. The control group consisted of 32 pregnant women without any abortions and with a history of at least one successfully terminated pregnancy. The serum levels of sCD26 and sCD30 and levels of IL-2, IL-4, IL-10, IL-13 and IFN