Résumé
Regarding the need for scientific development and achievement our national goals, it is clear that international cooperation has the main role in this way. Here is a report on what we have done during past almost 10 years [2001-2011] in the field of international medical research activities in Deputy Ministry for Research and Technology, Ministry of Health, Iran. Our effort was focused to identify and contact with the prominent scientific centers among the world where could make a connection between our researchers in medical science universities with those centers
Résumé
Neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. The aim of this study was to evaluate a PCR-based assay for specific and rapid detection of N. meninigitidis in CSF specimens. Since April 2002 to July 2006, 130 CSF specimens were collected from patients suspected of having baterial meningitis. Bacterial isolation and identification was carried out according to the standard bacteriological methods. The PCR was used to amplify a 101bp fragment of capsular transport gene A [ctr A] of N. meningitidis. PCR yielded an amplified product with the expected size of 101 base pair fragment. Sensitivity test proved 500 mg of N. meningitidis DNA as the final detection limit and specificity test revealed no cross-reaction for a wide range of respiratory pathogenic organisms. The PCR assay was more sensitive than the bacterial culturing. It might be possible to apply this procedure for rapid diagnosis of meningococci in clinical samples