Effect of honey on tracheobronchial tree of adult male guinea pigs

The effect of honey on the bronchoconstriction induced by histamine [0.5%] and acetylcholine [4%] aerosols in normal conscious guinea pigs, as well as its antianaphylactic effect on sensitized guinea pigs exposed to the antigen [5% ovalbumin aerosol] have been evaluated. Pretreatment with honey [3 g/kg] prolonged the preconvulsive time of normal conscious guinea pigs exposed to histamine, acetylcholine aerosols, or sensitized guinea pigs exposed to the antigen [5% ovalbumin] aerosol. The effect in the latter one is better than the first two types. This denoted that honey can be used for treatment of bronchial asthma due to its antagonistic effect to hypersensitivity reactions

Effect of cooling on contraction of sensitized guinea-pig trachea

The effect of cooling on contractility of ovalbumin sensitized guinea pig trachea was elucidated and the possible mechanisms underlying the effects of cooling on contractility induced by histamine, 5-HT and ovalbumin were investigated. Cooling of temperature from 37C to 25C is associated with significant increase in tracheal contractions to exogenously administered 5-1T, histamine and ovalbumin. Verapamil incubation [10 muM] produced significant depression of the contractile responses at 25C but not at 37C. These results demonstrated that the cooling induced contractile augmentation of the sensitized guinea-pig trachea to ovalbumin is due to 5-HT and histamine. The mechanisms of this augmentation may involve the increased opening of calcium channels in the membranes to allow calcium influx or the mobilization of calcium from intracellular calcium stores