In Silico Screening of Natural Products as Potential Inhibitors of SARS-CoV-2 Using Molecular Docking Simulation / 中国结合医学杂志

OBJECTIVE@#To explore potential natural products against severe acute respiratory syndrome coronavirus (SARS-CoV-2) via the study of structural and non-structural proteins of human coronaviruses.@*METHODS@#In this study, we performed an in-silico survey of 25 potential natural compounds acting against SARS-CoV-2. Molecular docking studies were carried out using compounds against 3-chymotrypsin-like protease (3CLPRO), papain-like protease (PLPRO), RNA-dependent RNA polymerase (RdRp), non-structural protein (nsp), human angiotensin converting enzyme 2 receptor (hACE2R), spike glycoprotein (S protein), abelson murine leukemia viral oncogene homolog 1 (ABL1), calcineurin-nuclear factor of activated T-cells (NFAT) and transmembrane protease serine 2.@*RESULTS@#Among the screened compounds, amentoflavone showed the best binding affinity with the 3CLPRO, RdRp, nsp13, nsp15, hACE2R. ABL1 and calcineurin-NFAT; berbamine with hACE2R and ABL1; cepharanthine with nsp10, nsp14, nsp16, S protein and ABL1; glucogallin with nsp15; and papyriflavonol A with PLPRO protein. Other good interacting compounds were juglanin, betulinic acid, betulonic acid, broussooflavan A, tomentin A, B and E, 7-methoxycryptopleurine, aloe emodin, quercetin, tanshinone I, tylophorine and furruginol, which also showed excellent binding affinity towards a number of target proteins. Most of these compounds showed better binding affinities towards the target proteins than the standard drugs used in this study.@*CONCLUSION@#Natural products or their derivatives may be one of the potential targets to fight against SARS-CoV-2.

efficacy of platelet rich plasma in resolution of plantar fasciitis in patients presenting with failed medical management

Objective: To assess the efficacy of platelet rich plasma intra-lesional injection in patients having planter fasciits

Methods: This descriptive case series study was conducted at department of orthopedic surgery, Services Hospital, Lahore for 10 monthes [ November 2015 to august 2016]. The non- probability: consecutive sampling technique was used. Demographic profile was also obtained. Intensity of pain was checked through visual analog pain scale. Then the patients were injected with 3 ml of autologous platelet rich plasma. The pain was noted using VAS scale after every 6 weeks up to 24 week. If pain was score on VAS, then efficacy was labeled. All the data was entered and analyzed on SPSS version 16

Results: In our study the mean age of the patients was 47.19 +/- 8.47 years and the male to female ratio of the patients was 1.6: 1. The mean age of the patients VAS score at 24th week was 1.38 +/- 1.41. In our study the efficacy achieved in 89[89%] patients

Conclusion: our study results concluded that is the use of platelet rich plasma intra- lesional injection is an effective management for pain control in patients with plantar fasciitis