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1.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 72-78, 2022.
Article Dans Chinois | WPRIM | ID: wpr-940290

Résumé

ObjectiveProteoglycan TPG-1 isolated from Trametes robiniophila(Huaier) has proved to have anti-hepatoma activity, and this paper aims to explore the molecular mechanism. MethodHuman hepatoma SK-HEP-1 cells were treated with TPG-1 (0, 0.05, 0.1, 0.25, 0.5, 1 g·L-1). Then cell survival was detected by methyl thiazolyl tetrazolium (MTT) and apoptosis by flow cytometry. In addition, expression of genes in SK-HEP-1 cells treated with or without TPG-1 was examined by DNA microarray to preliminarily explore the anti-hepatoma molecular mechanism of TPG-1. ResultTPG-1 inhibited the proliferation of SK-HEP-1 cells as compared with the blank group (P<0.01). After treatment with 1 g·L-1 TPG-1 for 48 h, the apoptosis rate of SK-HEP-1 cells increased (P<0.01), and TPG-1 promoted the cleavage of cysteinyl aspartate specific proteinase (Caspase)-3 and Caspase-7, the key mediators of apoptosis (P<0.01). Additionally, TPG-1 (1 g·L-1) suppressed the migration of SK-HEP-1 cells (P<0.05). A total of 971 differentially expressed genes (DEGs) were identified in SK-HEP-1 cells after treatment with TPG-1, with 486 up-regulated and 485 down-regulated. These DEGs were mainly involved in the Gene Ontology (GO) terms of interleukin-6 (IL-6) biosynthesis, antigen processing and presentation, superoxide dismutase activity, positive regulation of mitogen-activated protein kinase kinase kinase (MAPKKK) cascade, nature killer (NK) cell chemotaxis, and chemokine biosynthesis, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of nucleotide-binding oligomerization domain (NOD)-like receptor signaling pathway, apoptosis, Toll-like receptor signaling pathway, retinoic acid-inducible gene-Ⅰ (RIG-Ⅰ)-like receptor signaling pathway, T-cell receptor signaling pathway, and chemokine signaling pathway. Western blot results showed that TPG-1 (1 g·L-1) activated mitogen-activated protein kinase (MAPK) signaling pathway in SK-HEP-1 cells (P<0.01). ConclusionProteoglycan TPG-1 inhibited the proliferation and migration, and induced apoptosis of human hepatoma SK-HEP-1 cells. Up-regulation of MAPK signaling pathway may be responsible for the growth inhibition of human hepatoma SK-HEP-1 cells by TPG-1.

2.
National Journal of Andrology ; (12): 131-134, 2008.
Article Dans Chinois | WPRIM | ID: wpr-319278

Résumé

<p><b>OBJECTIVE</b>To discuss the diagnosis and treatment of non-specific granulomatous prostatitis (NSGP).</p><p><b>METHODS</b>Thirty-two cases of NSGP were diagnosed by puncture biopsy under transrectal ultrasound (TRUS) and treated with antibiotics and other medicines from September, 2000 to May, 2006.</p><p><b>RESULTS</b>Pathomorphologically, NSGP was basically characterized by granuloma with vessels or grand alveoli in the center. The mean follow-up was 24 months. Urination irritation and obstruction were improved. Q(max) was increased to 15.0-24.0 ml/s, and in 3 cases of urinary retention, to 12.0, 14.5 and 16.5 ml/s, respectively. Digital rectal examination (DRE) indicated a reduced size and softened texture of the prostate induration. PSA was decreased to 1.3-11.5 microg/L. Four cases experienced relapse but were cured after retreated. No prostate cancer was observed.</p><p><b>CONCLUSION</b>NSGP can be definitely diagnosed by puncture biopsy under TRUS and effectively relieved by antibiotics with the alpha-receptor blocker. In case of serious obstruction complicated by urinary retention, transurethral electrotomy can be considered.</p>


Sujets)
Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Humains , Mâle , Adulte d'âge moyen , Antagonistes alpha-adrénergiques , Utilisations thérapeutiques , Antibactériens , Utilisations thérapeutiques , Association de médicaments , Études de suivi , Granulome , Diagnostic , Imagerie diagnostique , Traitement médicamenteux , Prostatite , Diagnostic , Imagerie diagnostique , Traitement médicamenteux , Rectum , Échographie , Méthodes
3.
China Journal of Chinese Materia Medica ; (24): 1413-1415, 2008.
Article Dans Chinois | WPRIM | ID: wpr-264867

Résumé

<p><b>OBJECTIVE</b>To study the chemical constituents of an active fraction (DSS-A-N-30) from Danggui Shaoyao San.</p><p><b>METHOD</b>DSS-A-N30 was prepared by macroporous resin chromatography, the compound was isolated by column chromatography on silica gel and RPC-18, the structure was elucidated by spectroscopic methods.</p><p><b>RESULT</b>A new monoterpene glycoside was isolated and identified from DSS-A-N-30.</p><p><b>CONCLUSION</b>The new monoterpene glycoside was identified as 4"-hydroxyl-albiflorin.</p>


Sujets)
Composés pontés , Chromatographie sur gel , Médicaments issus de plantes chinoises , Chimie , Spectroscopie par résonance magnétique , Monoterpènes
4.
National Journal of Andrology ; (12): 693-695, 2007.
Article Dans Chinois | WPRIM | ID: wpr-232083

Résumé

<p><b>OBJECTIVE</b>To assess the application value of transrectal ultrasound (TRUS) in the diagnosis of chronic prostatitis.</p><p><b>METHODS</b>TRUS and examination of prostatic secretion (EPS) were used in the diagnosis of 3 500 cases of chronic prostatitis from September, 2000 to May, 2006.</p><p><b>RESULTS</b>Lower resonance of the inner gland, low-level echo, uneven echo light spots, incomplete outlines and unsmooth borderlines were found in 2279 cases (65.1%), and the enlarged prostate in 1 084 cases (31.0%), with clear integrated amicula and enhanced echogenic spots at the juncture of the external and inner gland. No obvious changes were noted in 137 cases (4.0%), and in another 391 cases (11.2%) were detected alteration of the acoustic image of cystospermitis and blurred margins and uneven echoes of the seminal vesicle. The WBC count in EPS was < 10/HP in 132 cases (3.8%), 10-19/HP in 2 156 cases (61.6%) and > or =20/HP in 1212 cases (34.6%).</p><p><b>CONCLUSION</b>TRUS, as a diagnostic means for chronic prostatitis, can be easily performed and causes little pain and therefore is readily accepted by patients. Combined with EPS, TRUS can provide more definite diagnostic evidence, and for those who are afraid of pain and reject EPS, it is a desirable alternative in the diagnosis of chronic prostatitis.</p>


Sujets)
Adulte , Humains , Mâle , Adulte d'âge moyen , Maladie chronique , Prostate , Imagerie diagnostique , Anatomopathologie , Prostatite , Diagnostic , Imagerie diagnostique , Rectum , Sensibilité et spécificité , Échographie , Méthodes
5.
Journal of Experimental Hematology ; (6): 530-533, 2003.
Article Dans Chinois | WPRIM | ID: wpr-278846

Résumé

The study was aimed to evaluate if the modified in situ cryopreservation could affect the biological function of mesenchymal stem cells (MSC) in vitro. Mesenchymal stem cells from human bone marrow were isolated by standard method and characterized with their morphology, cell-surface antigen profile and differentiation repertoire in vitro. The culture-expanded MSC were cryopreserved in situ with culture medium (DMEM-LG) containing 10% D MSO and 30% selected FCS in -70 degrees C. Following recovery of cryopreservation, differentiation to adipocytes, chondrocytes, and osteoblast in vitro and cell cycle analysis were performed to investigate whether the cryopreservation would change the differentiation potential of MSC. The results showed that after recovery of cryopreservation, there was no changes detected as compared with the culture-expanded MSC in both differentiation potency and growth pattern at 12 weeks. In conclusions: this optimized short term in situ cryopreservation at -70 degrees C could retain biological characteristics of human MSC for at least 3 months, and this method may be useful for cryopreservation of hum an bone marrow MSCs.


Sujets)
Humains , Cellules de la moelle osseuse , Biologie cellulaire , Cycle cellulaire , Différenciation cellulaire , Séparation cellulaire , Survie cellulaire , Cryoconservation , Cellules souches mésenchymateuses , Biologie cellulaire
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