Résumé
Objective:To analyze the effect of active lymphocyte immunotherapy (LIT) in patients with recurrent implantation failure (RIF), as well as its influence on serum galectin-3, galectin-1, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in cervical secretions.Methods:From January 2018 to March 2019, 144 patients with in-vitro fertilization and embryo transfer (IVF-ET) admitted to the First People′s Hospital of Tianmen were enrolled. Among them, there were 66 patients with RIF (RIF group) and 78 cases without RIF (non-RIF group). First, the differences of serum galectin-3, galectin-1, TNF-α and IL-6 in cervical secretions were compared between the two groups. Subsequently, the patients with RIF were treated with LIT, and the therapeutic effects were observed. The differences of the above indicators before and after treatment were compared.Results:The levels of serum galectin-1, galectin-3 and cervical secretion IL-6 in patients with RIF were lower than those in non-RIF patients [ovulation day: (35.94 ± 3.91) μg/L vs. (39.26 ± 3.75) μg/L, (53.10 ± 5.91) μg/L vs. (57.68 ± 6.87) μg/L, (73.13 ± 16.69) μg/L vs. (90.98 ± 16.25) μg/L; after ovulation for 6 d: (43.13 ± 4.53) μg/L vs. (48.95 ± 6.23) μg/L, (68.61 ± 7.70) μg/L vs. (72.25 ± 7.94) μg/L, (81.11 ± 16.02) μg/L vs. (91.21 ± 18.13) μg/L], the level of TNF-α in cervical secretion was higher than that in non-RIF patients [ovulation day: (13.33 ± 1.76) μg/L vs. (10.20 ± 1.74) μg/L; after ovulation for 6 d: (12.17 ± 1.64) μg/L vs. (9.36 ± 1.84) μg/L], and the differences were statistically significant ( P<0.05). After LIT treatment, the embryo implantation rate was 25.45% (42/165), the clinical pregnancy rate was 48.48% (32/66), and the abortion rate was 9.38% (3/32). After treatment, the serum galectin-1, galectin-3, cervical secretion IL-6 levels were higher than those before treatment, cervical secretions TNF-α levels were lower than before treatment, and the differences were statistically significant ( P<0.05). Conclusions:The levels of serum galectin-1, galectin-3, cervical secretions TNF-α, IL-6 in patients with RIF are different from those in non-RIF patients. LIT treatment can reduce the difference of these indicators and has certain treatment effect for patients with RIF.
Résumé
Objective To evaluate the effect of small interfering RNA targeting caspase-12 (caspase-12-siRNA) pretreatment on lung ischemia/reperfusion (I/R) injury in mice.Methods Forty male C57BL/6J mice,aged 6-8 weeks,weighing 16-24 g,were randomly allocated into 4 groups (n =10 each) using a random number table:sham operation group (group S),group I/R,negative control group (group NC) and caspase-12-siRNA pretreatment group (group siRNA).Lung I/R was induced by clamping the left pulmonary hilum for 30 min followed by 3 h reperfusion in anesthetized mice in IR,NC and siRNA groups.At 48 h before ischemia,negative control siRNA 20 μg and caspase-12-siRNA 20 μg were instilled intranasally in NC and siRNA groups,respectively,and the total volume was 50 μl.At 3 h of reperfusion,the animals were sacrificed and the left lung was removed for determination of wet/dry lung weight (W/D) ratio and lung water content in lung tissues and for microscopic examination.Pulmonary ultrastructure was examined with electron microscope.The quantitative evaluation index (QEI) for alveolar damage and apoptosis rate were calculated.Results Compared with group S,W/D ratio,lung water content,QEI for alveolar damage and apoptosis index were significantly increased in IR and NC groups,QEI for alveolar damage and apoptosis index were increased in group siRNA (P ＜ 0.05).Compared with IR and NC groups,W/D ratio,lung water content,QEI for alveolar damage and apoptosis index were significantly decreased (P ＜ 0.05),and the pathological changes of lungs were alleviated in group siRNA.There was no significant difference in the indices mentioned above between groups IR and NC (P ＞ 0.05).Conclusion Caspase-12-siRNA pretreatment can attenuate lung I/R injury in mice.