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1.
Braz. j. med. biol. res ; 40(11): 1455-1464, Nov. 2007. ilus, tab
Article Dans Anglais | LILACS | ID: lil-464306

Résumé

The retina is a highly differentiated tissue with a complex layered structure that has been extensively characterized. However, most of the previous studies focused on the histology of the central retina while little is known about the cellular composition, organization and function of the marginal retina. Recent research has identified a subpopulation of multipotential progenitor cells in the marginal regions of the retina, closest to the ciliary body ("ciliary marginal zone"). These cells are capable of differentiation in response to an appropriate stimulus. Thus, it is possible that the structure and composition of the marginal retina are distinct from those of the central retina to accommodate the potential addition of newly formed neurons. To characterize the cellular profile of the chick marginal retina, we labeled it immunohistochemically for markers whose staining pattern is well established in the central retina: calbindin, calretinin, protein kinase C, and choline acetyltransferase. Calbindin was present at very low levels in the marginal retina putative photoreceptor layer. Calretinin-positive horizontal cells were also sparse close to the ciliary marginal zone. The bipolar cells in the marginal outer plexiform layer were positive for anti-protein kinase C antibodies, but the density of labeling was also decreased in relation to the central retina. In contrast, the marginal starburst cholinergic amacrine cell pattern was very similar to the central retina. From these data we conclude that the structure of the marginal retina is significantly different from that of the central retina. In particular, the expression of late retina markers in the marginal retina decreased in comparison to the central retina.


Sujets)
Animaux , Corps ciliaire/cytologie , Protéines de l'oeil/analyse , Rétine/composition chimique , Cellules ganglionnaires rétiniennes/cytologie , Animaux nouveau-nés , Marqueurs biologiques/analyse , Prolifération cellulaire , Poulets , Choline O-acetyltransferase/analyse , Immunohistochimie , Protéine kinase C/analyse , Rétine/cytologie , Rétine/enzymologie , /analyse
2.
Braz. j. med. biol. res ; 39(3): 405-410, Mar. 2006. ilus
Article Dans Anglais | LILACS | ID: lil-421368

Résumé

To quantify the effects of methylmercury (MeHg) on amacrine and on ON-bipolar cells in the retina, experiments were performed in MeHg-exposed groups of adult trahiras (Hoplias malabaricus) at two dose levels (2 and 6 µg/g, ip). The retinas of test and control groups were processed by mouse anti-parvalbumin and rabbit anti-alphaprotein kinase C (alphaPKC) immunocytochemistry. Morphology and soma location in the inner nuclear layer were used to identify immunoreactive parvalbumin (PV-IR) and alphaPKC (alphaPKC-IR) in wholemount preparations. Cell density, topography and isodensity maps were estimated using confocal images. PV-IR was detected in amacrine cells in the inner nuclear layer and in displaced amacrine cells from the ganglion cell layer, and alphaPKC-IR was detected in ON-bipolar cells. The MeHg-treated group (6 µg/g) showed significant reduction of the ON-bipolar alphaPKC-IR cell density (mean density = 1306 ± 393 cells/mm²) compared to control (1886 ± 892 cells/mm²; P < 0.001). The mean densities found for amacrine PV-IR cells in MeHg-treated retinas were 1040 ± 56 cells/mm² (2 µg/g) and 845 ± 82 cells/mm² (6 µg/g), also lower than control (1312 ± 31 cells/mm²; P < 0.05), differently from the data observed in displaced PV-IR amacrine cells. These results show that MeHg changed the PV-IR amacrine cell density in a dose-dependent way, and reduced the density of alphaKC-IR bipolar cells at the dose of 6 µg/g. Further studies are needed to identify the physiological impact of these findings on visual function.


Sujets)
Animaux , Cellules amacrines/effets des médicaments et des substances chimiques , Poissons/métabolisme , Composés méthylés du mercure/toxicité , Parvalbumines/effets des médicaments et des substances chimiques , Protein kinase C-alpha/effets des médicaments et des substances chimiques , Cellules bipolaires rétiniennes/effets des médicaments et des substances chimiques , Cellules amacrines/métabolisme , Parvalbumines/métabolisme , Protein kinase C-alpha/métabolisme , Cellules bipolaires rétiniennes/métabolisme
3.
Braz. j. med. biol. res ; 23(10): 1051-5, 1990. ilus
Article Dans Anglais | LILACS | ID: lil-91651

Résumé

The present study was performed to map efferent projections of the pigeon intergeniculate leaflet (IGL) to other visual structures, with emphasis on the pathways containing neuropeptide Y (NPY). After injections of an anterograde tracer (Phaseolus vulgaris leucoagglutinin) into the IGL, labeled axons and presumptive terminals were seen in several retinorecipient and visually-related nuclei. All such areas contained immunoreactive fibers to antibodies against NPY. Electrolytic lesion of the IGL provoked a marked reduction in the number of NPY-labeled fibers in these visual structures. The data suggest that the IGL is the source of NPY-labeles axons which occur in many visual nuclei of the pigeon brain


Sujets)
Animaux , Corps géniculés/physiologie , Neuropeptide Y/métabolisme , Phytohémagglutinine , Voies optiques/physiologie , Columbidae , Neurones/physiologie
4.
Braz. j. med. biol. res ; 21(3): 649-52, Mar. 1988. tab
Article Dans Anglais | LILACS | ID: lil-60272

Résumé

Direction-selective units within the accessory optic system of the pigeon were shown to respond more strongly to motion along two main directions, downward-nasal and upward-temporal. Following ipsilateral telencephalic or pretectal lesions, these directions were modified in a systematic way. In the former, the principal response directions were downward-nasal and temporal and in the latter, downward-temporal and upward-temporal. These data indicate that the non-retinal afferents play an important role in the functional organization of the accessory optic system


Sujets)
Animaux , Mâle , Femelle , Neurones/physiologie , Tectum du mésencéphale/physiologie , Télencéphale/physiologie , Voies optiques/physiologie , Columbidae , Stimulation lumineuse
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