Résumé
Background: Hesa-A is a natural compound with anticancer properties. The exact mechanism of its action in esophageal cancer is not clear, yet. The aim of this study was to evaluate the cell toxicity effect of Hesa-A on the esophageal carcinoma cell lines, KYSE-30, and cell cycle genes expression
Methods: In this study, we tested cell toxicity with MTT [3-[4,5-Dirnethylthiazol-2-yl]-2,5-Diphenyltetrazolium Bromide] assay and flow cytometry to evaluate the cell cycle arrest
Real time polymerase chain reaction was used to assess the expression of P53, PI6, P21, cyclin Dl, and cyclin Bl genes
Results: Our results showed that Hesa-A is effective in the expression of cell cycling check point proteins. Hesa-A induced an arrest in G2 phase of esophageal cell cycle. The levels of P53 [>13 times], P21 [>21 times], PI6, cyclin Bl, and cyclin Dl genes were increased 48 hours after Hesa-A treatment
Conclusion: P21 and P16 expression were the potential mechanisms for G2 arrest of KYSE-30 esophageal cancer cell line by Hesa-A
Résumé
Bilirubin is the endproduct of heme catabolism. Serum bilirubin level can occationally be the only variable that affects the physician's decision. Bilirubin test is one of the most sensitive tests in the clinical laboratory. The aim of this research was to compare results of chemical versus direct spectrophotometric measurement of serum bilirubin. This cross-sectional study was carried out in 2006 on 119 specimens of serum. Bilirubin levels were determined by four methods: direct spectrophotometric by DAS and clinic II instruments, chemically with dichloroaniline [DCA], and diazo methods. Mean serum bilirubin measured by DAS, clinicII, diazo, and DCA methods was 8.38 [3.59], 7.80 [3.69], 9.70 [4.06], and 8.17 [4.09] mg/dl respectively. Difference of means was significant when examined using ANOVA [P<0.001]. The differences between diazo method and the other three methods were significant, while among the latter methods there was no statistically significant difference. Regarding significant differences between methods, for a better orientation of the physician and more accurate follow up of patients, we recommend to mention the method of measurement in laboratory reports