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Braz. j. microbiol ; 46(2): 433-441, Apr-Jun/2015. graf
Article Dans Anglais | LILACS | ID: lil-749723

Résumé

An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L−1. Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg−1) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D.


Sujets)
Cupriavidus/isolement et purification , Cupriavidus/métabolisme , Herbicides/métabolisme , /métabolisme , Acétates/métabolisme , Techniques bactériologiques , Biotransformation , Chine , Analyse de regroupements , Cupriavidus/génétique , Cupriavidus/physiologie , ADN bactérien/composition chimique , ADN bactérien/génétique , ADN ribosomique/composition chimique , ADN ribosomique/génétique , Concentration en ions d'hydrogène , Microscopie électronique à balayage , Données de séquences moléculaires , Phylogenèse , Propionates/métabolisme , Pyridines/métabolisme , Quinoxalines/métabolisme , /génétique , Analyse de séquence d'ADN , Température , Facteurs temps , Triticum
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