Evaluation of heat shock protein 70 in psoriasis

Psoriasis is a common chronic, relapsing, noninfectious inflammatory skin disease. The concept that psoriasis has a genetic basis has been accepted for many years and it is commonly thought of as a complex trait. Heat shock proteins [HSPs] are group of proteins whose expression is increased when the cells are exposed to elevated temperature or other stress.These proteins can be induced by a range of cellular stressors including increased temperature, oxidative stress and nutritional deficiencies. Hsps have been proposed to play an important role in the pathogenesis of psoriasis. The aim of this work is to detect the expression of HSP70 in psoriasis and its correlation to the disease severity and to review potential role of HSP70 in pathogenesis and therapy of psoriasis. Skin biopsies were taken from 20 patients with different severity of untreated chronic plaque-type psoriasis and from 20 healthy volunteers. Antibodies to HSP70 were analyzed immunohistochemically. Immunoreactivity intensity distribution index [IRIDI] scores including the proportion of immunoreactive cells and their staining intensity were calculated in the basal, suprabasal, superficial as well as the whole epidermal layers of patients and controls. Results of our study revealed that differential and total IRIDI scores for HSP70 expression showed highly significant higher values in psoriatic patients compared to controls. Statistical differences were found between the different groups of patients; according to their disease severity and controls. Positive correlations also existed between IRIDI scores of patients and disease severity. Our study provides further evidence on the importance of Hsp70 in the pathogenesis of psoriasis and shows increased Hsp70 expression in psoriatic epidermis correlated to increased severity of psoriasis. To our knowledge no previous studies made correlation with HSP70 expression in psoriasis and disease severity. Finally, we are looking forwards to the application of a new therapy that targets Hsp70 or its receptor CD91 and helps in treatment of psoriasis

relationship between placental growth factor, psoriasis and rheumatoid arthritis

Psoriasis is a chronic dermatosis characterized by the presence of heavy neutrophilic infiltrate in both the dermis and epidermis, together with elongated tortuous blood vessels in dermal papillae. Rheumatoid arthritis [RA] is a chronic inflammatory disease characterized by neovascularization and inflammatory cell infiltration within the synovium with associated synoviocyte hyperplasia. Several factors are found to be behind such a type of inflammation, including PIGF. Recognition of how this factor involved in the pathogenesis of psoriasis and RA may be of great help in the development of a new specific therapeutic modality of these disease. Is to study the role of PIGF [serum and synovial] as one of the factors underlying the pathogenesis of psoriasis and rheumatoid arthritis and its correlation with disease severity, duration and activity. Twenty eight RA patients were included in this study, diagnosed according to ACR classification. Forty psoriatic patients were also included in this study, examination and determination of the disease severity using PASI score were done. Measurement of the serum and synovial fluid [SF]PIGF level using ELISA technique was performed and correlation of disease duration and severity with its level was also done. We found that s.PIGF was high in serum of 64% of RA pts and in all synovial fluid samples. There is apositive correlation between the disease severity and its level ,however the correlation between the disease duration and the serum level of P1GF was insignificant.There is a positive correlation between serum and synovial PIGF in rheumatic patients. Serum PIGF [s.PIGF] was high in 92.5% of psoriatic patients and was significantly high in those with severe and moderate disease activity in comparison to those of mild activity and control group. s.PIGF was highly significant correlated with lesion severity. The prevalence of PsA is 35% of psoriatic pts,[male to female 5/2]. 8 patients have oligoarthritis, 6 patient have polyarthritis and DIP involvement .2 of them have spondyloarthropathy. PIGF has a role in the pathogenesis in rheumatoid arthritis and skin lesion of psoriasis and psoriatic arthritis