GC-MS and HPLC profiles of phenolic fractions of the leaf of Telfairia occidentalis

Telfairia occidentalis possesses high antioxidant activity. However, the antioxidant components of the plant have not yet been identified. This study was undertaken to identify the phenolics in the leaf of the plant. Extract and fractions of the leaf of the plant were analysed using the HPLC and GCMS. HPLC analysis revealed the presence of gallic acid [22.19microg/mg], catechin [29.17microg/mg], caffeic acid [9.17microg/mg], ferulic acid [0.94microg/mg], sinapic acid [1.91 microg/mg] and 4-hydroxy benzoic acid [43.86 microg/mg] in the aqueous extract. Phenolics fraction contained gallic acid [0.88 microg/mg], catechin [2.70microg/mg], caffeic acid [7.92microg/mg], ferulic acid [2.72microg/mg], benzoic acid [6.36microg/mg], pcoumaric acid [1.48microg/mg], quercetin [12.00microg/mg]. Only caffeic acid [2.50microg/mg], ferulic acid [0.44microg/mg] and quercetin [8.50microg/mg] were detected in the flavonoid fraction. While GCMS analysis showed the presence of methylparaben; ethylparaben; benzoic acid; 4-hydroxy-2-methoxy-3,5,6-trimethyl-, methyl ester; 4-hydroxy-3-methoxy; phenol, 5-methoxy-2-[methoxymethyl]-; phenol, 5-methoxy-2, 3- dimethyl; and phenol, 2-[2-benzothiazolyl]-. This study is the first to reveal the identity of some phenolics components of the leaf of Telfairia occidentalis

Antioxidant and cardio protective effect of palm oil leaves extract [standardized ethanolic fraction] in rats' model of saturated fats induced metabolic disorders

Recently, it is suggested to use POLE [palm oil leaf extract] as a nutraceutical health product in food industry due to its newly discovered content of polyphenols and antioxidant vitamins. In the experiment, the antioxidant and anti-lipid-peroxidation activities of the extract were confirmed using; DPPH [1-diphenyl-2-picryl-hydrazil] radical scavenging activity, ferric ion induced lipid peroxidation inhibition, reducing power and hydrogen peroxide scavenging activity assays. The cardio-protective activity was studied in vivo using a model of metabolic syndrome induced by high fat diet. Lipid profile, obesity indices, renal tubular handling of water and electrolytes, blood pressure and arterial stiffness were measured at the end of the treatment period. Sprague Dawley rats weighing 150-200 g were divided into six groups, viz; group C; was treated as a negative control and fed with standard rodents chow, group H; was treated as a positive control and fed with an experimental diet enriched with saturated free fatty acids for 8 weeks, groups HP0.5, HP1 and HP2 which were fed with 0.5,1 and 2 g/kg [body weight] /day of POLE orally during the last 24 days of the high fat diet feeding period and group P; fed with highest dose of POLE. Results revealed that POLE possesses a cardio-protective effect which is ascribed to its content of polyphenols.