Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 2 de 2
Filtre
1.
Journal of Zhejiang University. Science. B ; (12): 171-182, 2018.
Article Dans Anglais | WPRIM | ID: wpr-1010378

Résumé

The identification and use of molecular biomarkers have greatly improved the diagnosis and treatment of malignant tumors. However, a much deeper understanding of oncogenic proteins is needed for the benefit to cancer patients. The lipid raft marker proteins, flotillin-1 and flotillin-2, were first found in goldfish retinal ganglion cells during axon regeneration. They have since been found in a variety of cells, mainly on the inner surface of cell membranes, and not only act as a skeleton to provide a platform for protein-protein interactions, but also are involved in signal transduction, nerve regeneration, endocytosis, and lymphocyte activation. Previous studies have shown that flotillins are closely associated with tumor development, invasion, and metastasis. In this article, we review the functions of flotillins in relevant cell processes, their underlying mechanisms of action in a variety of tumors, and their potential applications to tumor molecular diagnosis and targeted therapy.


Sujets)
Animaux , Humains , Différenciation cellulaire , Endocytose , Protéines membranaires/physiologie , Tumeurs/étiologie , Régénération nerveuse
2.
Journal of Central South University(Medical Sciences) ; (12): 1012-1018, 2008.
Article Dans Chinois | WPRIM | ID: wpr-814159

Résumé

OBJECTIVE@#To determine the effect of activation of specific anti-tumor cytotoxic T lymphocytes (CTL) and the ability of cross-presentation in vitro by fusion of HLA-A2+ human dendritic cells (DCs) with HLA-A2- melanoma cells.@*METHODS@#The HLA-A2+ human dendritic cells and HLA-A2- melanoma cells were fused by PEG and were cultivated in complete RPMI1640 media containing FCS (10%) and GM-CSF for 24-48 h, and then co-cultured fusion cells with Melan-A specific T cells. HLA-A2- melanoma cells were negative control,While T2 cells and DC+Pts were positive control. The activation of anti-tumor CTL elicited by the fusion cells was detected by intracellular cytokine staining.@*RESULTS@#The immature DC could express CD80, CD83, CD86, HLA-DR, and HLA-ABC,but the mature DC induced by TNF-alpha, PGE-2, and CD40L further highly expressed above molecules. The rate of specific CTL cells primed by the fusion cells was 16.72%+/-4.26%, negative control was 0.21%+/-1.84%,and positive control was 28.60%+/-5.67%. The CTL from vaccine by fusing DC and LAR6 induced lysis of HLA-A2+ LAR1 cells.@*CONCLUSION@#The HLA-A2 restricted specific anti-tumor CTL can be induced in vitro by fusion of HLA-A2+ human dendritic cells with HLA-A2- melanoma cells.


Sujets)
Humains , Présentation d'antigène , Allergie et immunologie , Antigènes néoplasiques , Allergie et immunologie , Vaccins anticancéreux , Allergie et immunologie , Fusion cellulaire , Lignée cellulaire tumorale , Cellules dendritiques , Biologie cellulaire , Allergie et immunologie , Antigène HLA-A2 , Allergie et immunologie , Antigène MART-1 , Allergie et immunologie , Mélanome , Allergie et immunologie , Anatomopathologie , Lymphocytes T cytotoxiques , Allergie et immunologie
SÉLECTION CITATIONS
Détails de la recherche