Breast Tissue Reconstruction Using Polycaprolactone Ball Scaffolds in a Partial Mastectomy Pig Model

BACKGROUND@#Breast cancer patients suffer from lowered quality of life (QoL) after surgery. Breast conservancy surgery (BCS) such as partial mastectomy is being practiced and studied as an alternative to solve this problem. This study confirmed breast tissue reconstruction in a pig model by fabricating a 3-dimensional (3D) printed Polycaprolactone spherical scaffold (PCL ball) to fit the tissue resected after partial mastectomy. @*METHODS@#A 3D printed Polycaprolactone spherical scaffold with a structure that can help adipose tissue regeneration was produced using computer-aided design (CAD). A physical property test was conducted for optimization. In order to enhance biocompatibility, collagen coating was applied and a comparative study was conducted for 3 months in a partial mastectomy pig model. @*RESULTS@#In order to identify adipose tissue and fibroglandular tissue, which mainly constitute breast tissue, the degree of adipose tissue and collagen regeneration was confirmed in a pig model after 3 months. As a result, it was confirmed that a lot of adipose tissue was regenerated in the PCL ball, whereas more collagen was regenerated in the collagen-coated Polycaprolactone spherical scaffold (PCL–COL ball). In addition, as a result of confirming the expression levels of TNF-a and IL-6, it was confirmed that PCL ball showed higher levels than PCL–COL ball. @*CONCLUSION@#Through this study, we were able to confirm the regeneration of adipose tissue through a 3-dimensional structure in a pig model. Studies were conducted on medium and large-sized animal models for the final purpose of clinical use and reconstruction of human breast tissue, and the possibility was confirmed.

Acellular Dermal Matrix as a Core Strut for Projection in Nipple Reconstruction: Approaches for Three Different Methods of Breast Reconstruction

BACKGROUND: The objective of this paper was to describe a novel technique for improving the maintenance of nipple projection in primary nipple reconstruction by using acellular dermal matrix as a strut in one of three different configurations, according to the method of prior breast reconstruction. The struts were designed to best fill the different types of dead spaces in nipple reconstruction depending on the breast reconstruction method. METHODS: A total of 50 primary nipple reconstructions were performed between May 2012 and May 2015. The prior breast reconstruction methods were latissimus dorsi (LD) flap (28 cases), transverse rectus abdominis myocutaneous (TRAM) flap (10 cases), or tissue expander/implant (12 cases). The nipple reconstruction technique involved the use of local flaps, including the C-V flap or star flap. A 1×2-cm acellular dermal matrix was placed into the core with O-, I-, and L-shaped struts for prior LD, TRAM, and expander/implant methods, respectively. The projection of the reconstructed nipple was measured at the time of surgery and at 3, 6, and 9 months postoperatively. RESULTS: The nine-month average maintenance of nipple projection was 73.0%±9.67% for the LD flap group using an O-strut, 72.0%±11.53% for the TRAM flap group using an I-strut, and 69.0%±10.82% for the tissue expander/implant group using an L-strut. There were no cases of infection, wound dehiscence, or flap necrosis. CONCLUSIONS: The application of an acellular dermal matrix with a different kind of strut for each of 3 breast reconstruction methods is an effective addition to current techniques for improving the maintenance of long-term projection in primary nipple reconstruction.

Breakthrough Technique for Free Tissue Transfer of Poorly Vascularized Lower Extremity: Arteriovenous Loop Revisited

No abstract available.

Clinical Characteristic and Psychiatric Features of Self-Inflicted Wrist Laceration: A Single Institute Retrospective Study

PURPOSE: Self-inflicted wrist laceration is a common injury in the department of hand surgery. The aim of this study was to investigate the clinical characteristics and psychiatric features of self-inflicted wrist laceration using categorization according to wound severity. METHODS: We reviewed 71 patients from 2002 through 2012. All of the patients were grouped into four groups. Data regarding the following characteristics were collected: age, gender, size, structure involved, instruments used, history of previous self-inflicted injury, comorbidities in psychiatric and presentation of follow-up outpatient appointment to the department of plastic surgery and psychiatry. RESULTS: In these patients, approximately 64% of patients were female. About 80% of patients cut their wrist using a knife. And in grade 3-4 injury, percentage of glass injury was relatively high (22%), compared with other grades (3%). Unlike previous studies, patients in grade 3-4 tended to cut their wrist repeatedly. Focusing on psychiatric problems, approximately one quarter of patients had a previous history of self-infliction. In all patient groups, mood disorder was the most common disorder in patients who had a previous psychiatric disorder. But after operation, more than two thirds of patients had not visited department of psychiatry again. CONCLUSION: We identified some other differences among their characteristics. All patients in group also should be evaluated and surgically treated properly. A multidisciplinary approach is required for patients with wrist laceration due to self-injury in comparison to those with laceration due to other causes. Because many of them have previous self-injury experiences and psychiatric disease.

Thermal Injury During the Microvascular Free Flap: A Case Report

Thermal injury to free flap site can be severe and distressing injuries both for the patients and the surgeon. Thermal injuries to insensate free flaps are known complications often reported. But thermal injuries to free flaps are also occurred in the perioperative period. This study focused on the various factors associated with these injuries. We reported a 36-years old male patient with scalding burn in using warmed saline irrigation who underwent anterolateral thigh perforator free flap due to diabetic foot ulcer on dorsum of left foot. The clinical characteristics of thermal injuries during the free tissue transters are 1) Warmed saline irrigation is often too hot and a temperature excess 48degrees C should be considered very hot saline during the operation. 2) The direct exposure of the microscope light often causes iatrogenic burn, so copiously irrigating the surgical field and readjusting the light source's position or its output intensity are important. Surgeon must be aware that warmed saline irrigation & surgical microscope can cause thermal injury to free flap during the free tissue transfer.

Flow Measurement in the Hemodialysis Vascular Conduit for Surveillance of Internal Arteriovenous Fistula: A Trend Analysis

PURPOSE: We have already reported on a new method to calculate the intra-vascular conduit flow rate based on Bernoulli's theory for maintaining surveillance of the arteriovenous fistula (AVF) function. To assess the clinical validity of our methods, we examined a trend analysis on the calculated flow rate in hemodialysis vascular conduits. METHODS: From a total of 27 cases of native AVFs that were at least 3 months after construction, we measured the AVF flow rate (Q(D)) with Doppler ultrasonography first. When Q(D) was below 600 ml/min, a fistulogram was taken. The intra-vascular conduit static pressure (ps) was measured, and the flow rate (Q(a)) was calculated every month with using the mean arterial pressure. The patients with a decreased Q(a) of more than 10% over 3 months were referred for a diagnostic fistulogram. RESULTS: Twenty-seven AVFs were studied after 29.5+/-28.5 (4~120) months of operation. The mean Q(D) was 980.6+/-501.6 (144~2,230) ml/min. In 6 patients who showed a Q(D) less than 600 ml/min, 4 pathologic lesions were found on the diagnostic fistulogram: juxta-anastomosis stenosis (n=3) and a draining venous stenosis (n=1). Three patients who showed a Q(a) decrement were confirmed as having a draining venous stenosis. However, any unpredictable thrombosis with proximal stenosis (n=2) couldn't be detected. CONCLUSION: Q(a) represents the narrowing of the draining vein of an internal AVF. However, arterial or venous narrowing that is proximal to the arterial puncture site couldn't be detected with the calculated intra-conduit flow rate. The development of new methods that can detect proximal vascular stenosis and that can be used in combination with our method is anticipated in the near future.

Flow Measurement in the Vascular Access Conduit: an Ex Vivo Experimental Model

PURPOSE: Previously, we developed a new method to calculate the flow rate in the hemodialysis vascular conduit based on Bernoulli's theory for surveillance of the arteriovenous fistula (AVF) function. However, the calculated flow rate would be different from the true flow rate because due to various factors. To compare the true flow rate, with intra- conduit pressure, and the calculated flow rate, an ex vivo experimental model was developed. METHODS: The arterial end of the vascular conduit was connected to a saline-filled bottle, with the venous end connected to a flow meter to control the flow rate. By monitoring the change in the true flow rate (Q) with the flow meter, each arterial and venous static pressure (pA, pV) and total pressure (pT) were observed. Using these parameters, the intra-conduit flow rates (QA, QV) were calculated by Bernoulli's equation. Finally, we compared the pA or pV with Q, and calculated the difference between the QA or QV and Q. RESULTS: There were no statistical differences between any of the pressure measurement during the 5 consecutive 5 experiments (P<0.05). The static pressure (pA or pV) was closely correlated with Q (pA, R2=0.950, P=0.000; pV, R2= 0.952, P=0.000). The calculated flow rate (QA or QV) was not in complete in accord with Q, but was closely correlated (QA, R2=0.961, P=0.000; QV, R2=0.961, P=0.000). CONCLUSION: The pressure measurement and calculated flow rate indicate the nature of the true flow rate in the vascular conduit.