non reponse a la vaccination antihepatitique chez le personnel soignant

The authors report the results of an investigation of witness cases realized in collaboration between occupational medecine service and immunology laboratory of hedi CHAKER university hospital SFAX during the year 2000. the purpose was to seartch the genetic control of the HLA class l system for the non response to hepatitis B vaccine and to evaluate the contribution of other favorite factors as tabac, sex age. Thus, in a population of 32 healthy agents found nonresponders to hepatitis B vaccine by the titers of anti HBs antibody, we have performed the HLA-A, -B phenotypes by the technique of complement dependent microcytotoxicity. The frequency of studied HLA class l antigens, was compared for the non responders group, to the frequency observed in witnesses group, done with 52 responders health agents. The rate of nonresponsiveness hepatitis B vaccine was evaluated up to 5%. Statistically significant difference was observed for HLA A1 and B44 markers showing frequencies which were considerably higher in the non responders than in witnesses

Prevalence of CMV antibodies in blood donors of sfax region

Detection of anti-CMV antibodies was carried out in sera of healthy blood donors, divided into groups of 20 according to age and sex. Sera were tested for anti-CMV by an ELISA test [Enzygnost anti-CMV/IgG-Behring]. Among 280 sera, 272 were positive for IgG to CMV [97.14 percent]. The prevalence of those antibodies was high in all age stratum [95-110 percent] but was higher in women than in men [98.57 percent versus 95.71 percent]. The titre of IgG to CMV was superior to 12 IL/mL in 56.43 percent of CMV positive donors. So, the leucocyle removal is the only alternative for the prevention of post-transfusional CMV infection. The high percentage of donors with anti-CMV antibodies level more than to 12 UL/mL allow to consider the use of plasmapheresis for preparing specific immunoglobulins to CMV